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> home > Products > Antibodies > Biological Process > Post-translational Protein Modification > Cdc25C Antibody (C-term)

Cdc25C Antibody (C-term)Rabbit Monoclonal Antibody

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Catalog #	Size	Availability	Price
AJ1175b	100ul 400 ul	2-3 days	$ 315.00	DISCONTINED INQUIRE CLICK INQUIRE Add to cart

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Cdc25C Antibody (C-term) - Product info
Application	WB Applications Legend: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Primary Accession	P30307
Reactivity	Human
Clone Names	E303
Calculated MW	53365 Da
Gene ID 995
Other Names CDC25C, M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C
Target/Specificity A synthetic peptide corresponding to residues in C-terminus of human Cdc25C was used as immunogen. The antibody does not cross-react with other MPI phosphatase family members.
Dilution WB~~1:500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions Cdc25C Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Cdc25C Antibody (C-term) - Protein Information
Name CDC25C
Function Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity
Cellular Location Nucleus.

Cdc25C Antibody (C-term) - Related products

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LY11518a: CDC25C Over-expression Lysate

BP7862b: CDC25C Antibody (C-term) Blocking Peptide

BP7862d: CDC25C Antibody (Center) Blocking Peptide

AT1458a: CDC25C Antibody (monoclonal) (M01)

AO1357a: CDC25C Antibody

AJ1175a: Cdc25C Antibody

AJ1175b: Cdc25C Antibody (C-term)

AJ1175c: Cdc25C Antibody Phospho (pS216)

Cdc25C Antibody (C-term) - Application data

A. Western blot analysis on A431 cell lysate using anti-Cdc25C (C-term) RabMAb (Cat. #AJ1175b); dilution 1:500.

Cdc25C Antibody (C-term) - Research Areas

Antibodies Biological Process Post-translational Protein Modification Phosphate Metabolic Process Phosphorus Metabolic Process Protein Modification Process Biopolymer Modification Metabolic Process Primary Metabolic Process Protein Metabolic Process Cellular Protein Metabolic Process Cellular Metabolic Process Cellular Process Regulation Of Phosphorylation Regulation Of Phosphate Metabolic Process Regulation Of Phosphorus Metabolic Process Macromolecule Metabolic Process Regulation Of Kinase Activity Regulation Of Protein Kinase Activity Regulation Of Transferase Activity Cellular Macromolecule Metabolic Process Regulation Of Molecular Function Regulation Of Catalytic Activity Regulation Of Cell Cycle Interspecies Interaction Between Organisms Cell Proliferation Protein Amino Acid Dephosphorylation Regulation Of Metabolic Process Cellular Component Organization Interphase Of Mitotic Cell Cycle Mitotic Cell Cycle Interphase Regulation Of Mitotic Cell Cycle

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

Cdc25C is a tyrosine protein phosphatase required for progression of the cell cycle. Cdc25C activates cell cycle-specific cyclin-dependent kinases (CDKs), including cdc2, via dephosphorylation events (1,2). Cdc25C is activated via phosphorylation at Ser216 (3). In response to DNA damage and DNA replicational stress, Chk1 and Chk2 phosphorylate Cdc25C to prevent entry into mitosis (4,5).

REFERENCES

1. Sadhu, K., et al. Human homolog of fission yeast cdc25 mitotic inducer is predominantly expressed in G2. Proc Natl Acad Sci U S A. 87: 5139
2. Jessus, C. and R. Ozon. Function and regulation of cdc25 protein phosphate through mitosis and meiosis. Prog. Cell Cycle Res. 1: 215
3. Peng, C.Y., et al. Mitotic and G2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216. Science 277: 1501
4. Blasina, A., et al. A human homologue of the checkpoint kinase Cds1 directly inhibits Cdc25 phosphatase. Curr Biol. 9: 1
5. Matsuoka, S., et al. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science 282: 1893-7 (1998).