|Calculated MW||62720 Da|
|Other Names||Cell division control protein 6 homolog, CDC6-related protein, Cdc18-related protein, HsCdc18, p62(cdc6), HsCDC6, CDC6, CDC18L|
|Target/Specificity||A phospho-specific peptide corresponding to residues surrounding Serine 106 of human Cdc6 was used as an immunogen. The antibody only detects Cdc6 phosphorylated on Serine 106.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Cdc6 Antibody Phospho (pS106) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.|
|Cellular Location||Nucleus. Cytoplasm. Note=The protein is nuclear in G1 and cytoplasmic in S-phase cells (PubMed:9566895)|
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Provided below are standard protocols that you may find useful for product applications.
The Cdc6 protein is an essential component of pre-replication complexes (preRCs), which assemble at origins of DNA replication during the G1 phase of the cell cycle (1). Cdc6 participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated (2). It has been concluded that expression of Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells (3). In vitro, Cdc6 is an excellent substrate for Cdk2, phosphorylated at three sites (Serine 54, Serine 74 and Serine 106) (4).
1. Hall JR, et al. Mol Biol Cell. 18(9):3340-50, 2007
2. Williams RS, et al. Proc Natl Acad Sci U S A. 94(1):142-7,1997
3. Yan Z, et al. Proc Natl Acad Sci U S A. 95(7):3603-8, 1998
4. Jiang W, et al. Proc Natl Acad Sci U S A. 96(11):6193-8,1999
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