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Cdc6 Antibody Phospho (pS54)Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1176b | 100ul 400 ul | 2-3 days | $ 355.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
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Cdc6 Antibody Phospho (pS54) - Product info | |
| Application | WB, IF
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| Primary Accession | Q99741 |
| Reactivity | Human |
| Clone Names | EPR759Y |
| Calculated MW | 62720 Da |
| Gene ID 990 | |
| Other Names CDC6, CDC18L, Cell division control protein 6 homolog, CDC6-related protein;p62(cdc6) | |
| Target/Specificity A phospho-specific peptide corresponding to residues surrounding serine 54 of human Cdc6 was used as an immunogen. The antibody only detects Cdc6 phosphorylated on Serine 54. | |
| Dilution WB~~1:1000~2000 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Cdc6 Antibody Phospho (pS54) is for research use only and not for use in diagnostic or therapeutic procedures. | |
Cdc6 Antibody Phospho (pS54) - Protein Information | |
| Name CDC6 | |
| Synonyms CDC18L | |
| Function Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated | |
| Cellular Location Nucleus. Cytoplasm. Note=The protein is nuclear in G1 and cytoplasmic in S-phase cells | |
Cdc6 Antibody Phospho (pS54) - Related products
AP3058a: Phospho-CDC6-S54 Antibody
AP6271a: Cdc6 Antibody (N-term)
BP3058a: Phospho-CDC6-S54 Antibody Blocking Peptide
BP6271a: Cdc6 Antibody (N-term) Blocking Peptide
Cdc6 Antibody Phospho (pS54) - Application data
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A.Western blot analysis on (A) Raji and (B) Raji + FBS cell lysates using anti-Phospho-Cdc6 (pS54) RabMAb (Cat. #AJ1176b), 1:2000 dilution. Cells were either (A) untreated (B) treated with FBS
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B. Immunofluorescent staining of HeLa cells using Phospho-specific anti-CDC6 RabMAb (Cat. #AJ1176b).
Cdc6 Antibody Phospho (pS54) - Research Areas
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BACKGROUND
The Cdc6 protein is an essential component of pre-replication complexes (preRCs), which assemble at origins of DNA replication during the G1 phase of the cell cycle (1). Cdc6p and cdc18+ are unstable proteins that are essential and rate limiting for the initiation of DNA replication in Saccharomyces cerevisiae and Schizosaccharomyces pombe, respectively. They participate in checkpoint controls that ensure DNA replication is completed before mitosis is initiated (2). It has been concluded that expression of Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells (3). Cdc6 is believed to be phosphorylated at Serine 54 during S phase and functions as a chromatin-bound signal that prevents reformation of prereplication complexes (4).
REFERENCES
1. Hall JR, et al. Mol Biol Cell. 18(9):3340-50, 2007
2. Williams RS, et al. Proc Natl Acad Sci U S A. 94(1):142-7, 1997
3. Yan Z, et al. Proc Natl Acad Sci U S A. 95(7):3603-8, 1998
4. Alexandrow MG, et al. Molecular and Cellular Biology 24(4): 1614-1627, 2004
