- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
Cdk2 Antibody (C-term)Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AJ1177a | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Cdk2 Antibody (C-term) - Product info | |
| Application | WB, IF
|
| Primary Accession | P24941 |
| Reactivity | Human, Mouse |
| Clone Names | E304 |
| Calculated MW | 33930 Da |
| Gene ID 1017 | |
| Other Names CDK2, Cell division protein kinase 2, p33 protein kinase | |
| Target/Specificity A synthetic peptide corresponding to residues in C-terminus of human Cdk2 was used as immunogen. | |
| Dilution WB~~1:2000 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Cdk2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
Cdk2 Antibody (C-term) - Protein Information | |
| Name CDK2 | |
| Synonyms CDKN2 | |
| Function Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT- mediated activation of histone gene transcription during S phase Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization | |
| Cellular Location Cytoplasm, cytoskeleton, centrosome. Nucleus, Cajal body. Cytoplasm. Endosome. Note=Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3) | |
Cdk2 Antibody (C-term) - Related products
AP3067a: Phospho-Cdk2-T160 Antibody
AP3500a: Phospho-CDK2-pT41 Antibody
AP3613a: Phospho-CDK2-pT14 Antibody
AP7518b: CDK2 Antibody (C-term)
AP7518c: CDK2 Antibody (Center R199)
LY10013a: CDK2 Over-expression Lysate
BP3067a: Phospho-Cdk2-T160 Antibody Blocking Peptide
BP3500a: Phospho-CDK2-pT41 Antibody Blocking Peptide
BP3613a: Phospho-CDK2-pT14 Antibody Blocking Peptide
BP7518b: CDK2 Antibody (C-term) Blocking Peptide
BP7518c: CDK2 Antibody (Center R199) Blocking Peptide
BP7518d: CDK2 Antibody (T14) Blocking Peptide
AJ1177a: Cdk2 Antibody (C-term)
Cdk2 Antibody (C-term) - Application data
-
A. Western blot analysis on HeLa cell lysate using anti-Cdk2 (C-term) RabMAb (Cat. #AJ1177a); dilution 1:5,000.
-
B. Immunofluorescent staining of HeLa cells using anti-Cdk2 (C-term) RabMAb (Cat. #AJ1177a).
Cdk2 Antibody (C-term) - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Cyclin-dependent protein kinase 2 (Cdk2), also known as cell division protein kinase 2, is a 33-kDa nuclear protein that regulates events within G1 and S phase in the human cell cycle (1-4). Cdk2 is inactive until a complex is formed with cyclin A, D, or E (2). Inhibitory phosphorylation occurs on Thr14 and Tyr15. Activation of the Cdk2 complex requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160, which is mediated by CAK (3,4).
REFERENCES
1. Morgan, D.O. Principles of CDK regulation. Nature 374: 131
2. Elledge, S.J., et al. CDK2 encodes a 33-kDa cyclin A-associated protein kinase and is expressed before CDC2 in the cell cycle. Proc. Natl. Acad. Sci. USA 89: 2907
3. Gu, Y., et al. Cell cycle regulation of CDK2 activity by phosphorylation of Thr160 and Tyr15. EMBO J. 11: 3995
4. Fesquet, D., et al. The MO15 gene encodes the catalytic subunit of a protein kinase that activates cdc2 and other cyclin-dependent kinases (CDKs) through phosphorylation of Thr161 and its homologues. EMBO J. 12: 3111
