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CDK9 Antibody

Rabbit Monoclonal Antibody

  • WB - CDK9 Antibody AJ1180b
    A.Western blot analysis on (A) HT-29, (B) HeLa and (C) 293T cell lysates using anti-CDK9 RabMAb (Cat. #AJ1180b), dilution 1:2000.
  • IHC - CDK9 Antibody AJ1180b
    B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-CDK9 RabMAb (Cat. #AJ1180b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P50750
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP3118
Calculated MW 42778 Da
Gene ID 1025
Other Names Cyclin-dependent kinase 9, C-2K, Cell division cycle 2-like protein kinase 4, Cell division protein kinase 9, Serine/threonine-protein kinase PITALRE, Tat-associated kinase complex catalytic subunit, CDK9, CDC2L4, TAK
Target/Specificity A synthetic peptide corresponding to residues in human CDK9 was used as an immunogen.
Dilution WB~~1:2000~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCDK9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDK9
Synonyms CDC2L4, TAK
Function Protein kinase involved in the regulation of transcription. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP. This complex is inactive when in the 7SK snRNP complex form. Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR, and the negative elongation factors DSIF and NELF. Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling). Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis. P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export. Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3; integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing. The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro. Replication stress response protein; the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single- stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage. In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6. Promotes cardiac myocyte enlargement. RPB1/POLR2A phosphorylation on 'Ser-2' in CTD activates transcription. AR phosphorylation modulates AR transcription factor promoter selectivity and cell growth. DSIF and NELF phosphorylation promotes transcription by inhibiting their negative effect. The phosphorylation of MYOD1 enhances its transcriptional activity and thus promotes muscle differentiation.
Cellular Location Nucleus. Cytoplasm. Nucleus, PML body. Note=Accumulates on chromatin in response to replication stress Complexed with CCNT1 in nuclear speckles, but uncomplexed form in the cytoplasm. The translocation from nucleus to cytoplasm is XPO1/CRM1-dependent. Associates with PML body when acetylated
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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Cyclin-dependent kinase 9, CDK9, is a member of the cell division cycle 2 (CDC2)-like family of kinases with roles in different cellular processes such as signal transduction, basal transcription, HIV-Tat- and MyoD-mediated transcription and differentiation. The cyclin-dependent kinase pair CDK9/cyclin T1 complex, also called positive transcription elongation factor b (P-TEFb), is very important in differentiation of several cell types, possibly including lymphoid cells (1-2). The association of CDK9 with the HIV-Tat protein suggests a possible involvement in AIDS, and its responsiblility for the kinase activity associated with the TAK and P-TEFb complexes suggests its involvement in the transcription process (3).


1. Bellan C, et al. Journal of pathology 203(4):946-952, 2004
2. Simone C, et al. Front Biosci. 6:D1073-82, 2001
3. Giulia De Falco, et al. Journal of Cellular Physiology 177(4):501 - 506, 1998

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Cat# AJ1180b
(40 western blots)
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