|Application ||WB, IF|
|Calculated MW||15991 Da|
|Other Names||Histone H3-like centromeric protein A, Centromere autoantigen A, Centromere protein A, CENP-A, CENPA|
|Target/Specificity||A synthetic peptide corresponding to residues in the C-term of human CENP-A was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CENP-A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. The CENPA-H4 heterotetramer can bind DNA by itself (in vitro).|
|Cellular Location||Nucleus. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height|
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Centromere protein A (CENP-A) is an 18-kDa centromere-specific histone variant with 62% amino acids homology to the C-terminal of histone H3. Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. The depletion of histone H3 at the CENP-A binding domain suggests CENP-A to be a possible replacement for histone H3 in the packaging process of alpha-satellite DNA into primary chromatin structure (2). CENP-A is essential in the formation of specialized nucleosomes at the centromere, implicating CENP-A as a centromere-specific epigenetic marker (3).
1. Warburton PE, Cooke CA, et al. Curr Biol 7:901-904, 1997.
2. Yokda K, et al. Proc Natl Acad Sci USA 97:7266-7271, 2000.
3. Choo KHA, et al. Cell Biol 10:182
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