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CENP-A Antibody (C-term)

Rabbit Monoclonal Antibody

     
  • WB - CENP-A Antibody (C-term) AJ1182a
    A. Western blot analysis on HeLa cell lysate using anti-CENP-A RabMAb (Cat. #AJ1182a), dilution 1:20,000
  • IF - CENP-A Antibody (C-term) AJ1182a
    B. Immunofluorescent staining of HeLa cells using anti-CENP-A RabMAb (Cat. #AJ1182a).
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession P49450
Reactivity Human, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP800Y
Calculated MW 15991 Da
Gene ID 1058
Other Names Histone H3-like centromeric protein A, Centromere autoantigen A, Centromere protein A, CENP-A, CENPA
Target/Specificity A synthetic peptide corresponding to residues in the C-term of human CENP-A was used as an immunogen.
Dilution WB~~1:100000~200000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCENP-A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CENPA
Function Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes (PubMed:7962047, PubMed:9024683, PubMed:11756469, PubMed:14667408, PubMed:15702419, PubMed:15475964, PubMed:15282608, PubMed:17651496, PubMed:19114591, PubMed:27499292, PubMed:20739937). Replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore (PubMed:18072184). The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3 (PubMed:27499292, PubMed:26878239). May serve as an epigenetic mark that propagates centromere identity through replication and cell division (PubMed:15475964, PubMed:15282608, PubMed:26878239, PubMed:20739937, PubMed:21478274). Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis (PubMed:11756469, PubMed:14667408, PubMed:18072184, PubMed:23818633, PubMed:25556658, PubMed:27499292).
Cellular Location Nucleus Chromosome, centromere, kinetochore Chromosome, centromere. Note=Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height (PubMed:19114591). Phosphorylation at Ser-68 during early mitosis abolishes association with chromatin and centromeres and results in dispersed nuclear location (PubMed:25556658).
Citations (0)

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Background

Centromere protein A (CENP-A) is an 18-kDa centromere-specific histone variant with 62% amino acids homology to the C-terminal of histone H3. Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. The depletion of histone H3 at the CENP-A binding domain suggests CENP-A to be a possible replacement for histone H3 in the packaging process of alpha-satellite DNA into primary chromatin structure (2). CENP-A is essential in the formation of specialized nucleosomes at the centromere, implicating CENP-A as a centromere-specific epigenetic marker (3).

References

1. Warburton PE, Cooke CA, et al. Curr Biol 7:901-904, 1997.
2. Yokda K, et al. Proc Natl Acad Sci USA 97:7266-7271, 2000.
3. Choo KHA, et al. Cell Biol 10:182

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Discontinued
Cat# AJ1182a
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