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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   c-Jun Antibody Phospho (pT93)   

c-Jun Antibody Phospho (pT93)

Rabbit Monoclonal Antibody

  • WB - c-Jun Antibody Phospho (pT93) AJ1188f
    A. Western blot analysis on NIH/3T3 cell lysates using anti-Phospho-c-Jun (pT93) RabMAb (Cat. #AJ1188f), 1:5000 dilution. Cells were either (A) untreated (B) treated with Anisomycin
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05412
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names EPR2237
Calculated MW 35676 Da
Gene ID 3725
Other Names Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, JUN
Target/Specificity A synthetic peptide corresponding to residues surrounding Threonine 93 of human c-Jun was used as an immunogen. The antibody only detects c-Jun phosphorylated at Threonine 93.
Dilution WB~~1:1000~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsc-Jun Antibody Phospho (pT93) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name JUN
Function Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Cellular Location Nucleus.
Research Areas
Citations (0)

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c-Jun is a major component of the heterodimeric transcription factor AP-1 and is essential for embryonic development (1). It mediates several cellular processes, including proliferation and survival, and is upregulated in many carcinomas (2). Transactivation of c-Jun is regulated by Jun-N-terminal Kinases (JNKs) through phosphorylation at Serine 63 and 73, as well as at threonine 91 and 93 (3). Evidence supports that phosphorylation in NH2-terminal residues of c-Jun stimulates the dephosphorylation of the COOH-terminal sites, and consequently increases the DNA-binding activity of the transcription factor (4). c-Jun N-terminal kinase (JNK) isoforms are required for the phosphorylation of Threonine 91 and 93 in response to anisomycin in macrophages and TNF-alpha or anisomycin in fibroblasts (5).


1. Behrens A, et al. Nat Genet. 21(3):326-9, 1999
2. Eferl R, et al. Cell. 112(2):181-92, 2003
3. Weiss C., et al. EMBO J. 22(14): 3686-95, 2003
4. Papavassiliou, A.G., et al. EMBO J. 14(9):2014-2019, 1995
5. Morton S., et al. The EMBO Journal 22, 3876-3886, 2003

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Cat# AJ1188f
(40 western blots)
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