|Application ||WB, IHC|
|Calculated MW||109865 Da|
|Other Names||Mast/stem cell growth factor receptor Kit, SCFR, Piebald trait protein, PBT, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, CD117, KIT, SCFR|
|Target/Specificity||A synthetic peptide corresponding to residues in the C-terminus (cytoplasmic domain) of human c-Kit was used as an immunogen. The antibody does not cross-react with other CSF-1/PDGF receptor family member.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||c-Kit/CD117 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.|
|Cellular Location||Isoform 1: Cell membrane; Single-pass type I membrane protein Isoform 3: Cytoplasm. Note=Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head|
|Tissue Location||Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells|
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Provided below are standard protocols that you may find useful for product applications.
Member of the Tyrosine Kinase Receptor (TKRs) and highly homologous to receptor PDF and CSF-1 (1-2), c-Kit is a cellular homologue of the oncogene v-Kit of the Hardy Zuckerman 4-feline sarcoma virus (3). Activation of c-Kit tyrosine kinase by SCF (Stem Cell factor) leads to autophosphorylation and association of c-Kit with substrate PI3K (4). Missense substitution within the cellular tyrosine kinase may cause Piebaldism an autosomal dominant disorder of the skin (5).
1. Yarden, Y., et al. (1987) EMBO (Eur. Mol. Biot. Organ.) J. 6:3341-3351.
2. Qiu, F. H.,et al. (1988) EMBO (Eur. Mol. Biot. Organ.) J. 7:1003-1011.
3. Besmer, et al. 1986. Nature (Lond.). 320:415-421.
4. Zsebo, K. M., et al. (1990) Cell. 63:195-201.
5. Spritz RA, Giebel LB, Holmes SA. (1992) Am J Hum Genet. Feb;5 (2):261-9.
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