|Calculated MW||284539 Da|
|Other Names||Spectrin alpha chain, non-erythrocytic 1, Alpha-II spectrin, Fodrin alpha chain, Spectrin, non-erythroid alpha subunit, SPTAN1, NEAS, SPTA2|
|Target/Specificity||A synthetic peptide corresponding to residues in human Alpha Fodrin was used as an immunogen. This antibody only detects the large fragment of Alpha Fodrin resulting from cleavage at aspartic acid 1185.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Cleaved alpha-Fodrin Antibody (Asp1185) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.|
|Cellular Location||Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.|
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Provided below are standard protocols that you may find useful for product applications.
Alpha Fodrin is a member of the spectrin family, which are widely distributed filamentous proteins that form a supporting and organizing scaffold for cell membranes (1). Non-erythroid alpha spectrin, also called Alpha Fodrin, is a major cortical cytoskeletal protein that has been implicated in the establishment of specialized membrane- cytoskeletal domains in differentiating cells (2-3). Cleavage of Alpha Fodrin accompanies apoptosis, induced by activation via the CD3/T cell receptor complex, ligation of the Fas (CD95) molecule and other Fas-expressing cells, or treatment of cells with staurosporine, dexamethasone, or synthetic ceramide (4). The major products of the cleavage are amino-terminal (135 kDa) and carboxy-terminal (150 kDa and 120 kDa) fragments. Antibodies against Alpha Fodrin are observed in diseases characterized by chronic apoptosis, and are valuable in the evaluation fo Sicca- and Sjogren's syndrome (5-6).
1. Leto TL, et al. Mol Cell Biol. 8(1):1-9, 1988 2. Simonovic M, et al. J Biol Chem. 281(45):34333-40, 2006 3. DH Giebelhaus, et al. The Journal of Cell Biology 105:843- 853,1987 4. Martin SJ, et al. J Biol Chem. 270(12):6425-8, 1995 5. Ulbricht K U, et al. Autoimmunity Reviews 2(2):109- 113,2003 6. Kahaly, G. J., et al. Clinical & Experimental Immunology 140:166-172(7), 2005
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