|Application ||WB, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||47873 Da|
|Other Names||COP9 signalosome complex subunit 3, SGN3, Signalosome subunit 3, JAB1-containing signalosome subunit 3, COPS3, CSN3|
|Target/Specificity||A synthetic peptide corresponding to residues at the C-terminus of human COPS3 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||COPS3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Widely expressed. Expressed at high level in heart and skeletal muscle.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
COPS3 is a component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. Individual subunits of the complex have been linked to various signal transduction pathways leading to gene expression and cell cycle control (1, 2). Subunit 3 (COPS3) possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-B alpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation (3). COPS3 is suggested as a specific negative regulator of TNF-induced NF-kappaB activation pathways because its over-expression inhibits NF-kappaB activation (4).
1. Tsuge T, et al. J Mol Biol. 305(1):1-9, 2001
2. Fang L., et al. J. Proteome Res. 7:4914-4925, 2008
3. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 36:D190-D195, 2008
4. Hong X, et al. FEBS Lett. 499(1-2):133-6, 2001
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.