|Application ||WB, IHC|
|Calculated MW||16693 Da|
|Other Names||Protein phosphatase 1 regulatory subunit 14A, 17 kDa PKC-potentiated inhibitory protein of PP1, Protein kinase C-potentiated inhibitor protein of 17 kDa, CPI-17, PPP1R14A, CPI17, PPP1INL|
|Target/Specificity||A synthetic peptide corresponding to residues in the N-term of human CPI-17 aplha was used as immunogen. This antibody may also detect isoform CPI-17 beta.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CPI-17 alpha Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.|
|Tissue Location||Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
PKC Potentiated Inhibitor Protein-17 (CPI-17) is a 17 kDa protein (1). Expressed in smooth muscles and neuronal cells, CPI-17 is a myosin phosphatase inhibitor protein. Phosphorylation of CPI-17 at Thr38 enhances its inhibitory effect by 100 fold (2). Once phosphorylated, CPI-17 inhibits PP1c and MLCP holoenzyme activity (3).
1. Eto M, Ohmori T, Suzuki M, Furuya K & Morita F (1995). J Biochem 118, 1104-1107
2. Eto M, Senba S, Morita F & Yazawa M (1997). FEBS Lett 410, 356-360
3. Senba S, Eto M & Yazawa M (1999). J Biochem 125, 354-362
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.