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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Creatine Kinase B Antibody   

Creatine Kinase B Antibody

Rabbit Monoclonal IgG

  • WB - Creatine Kinase B Antibody AJ1198a
    Western blot analysis on (A) SHSY-5Y (B) Hela (C) Y-79 (D) SW840 cell lysates using anti-Creatine Kinase B RabMAb (Cat. #AJ1198a).
  • IHC - Creatine Kinase B Antibody AJ1198a
    Immunohistochemical analysis of paraffin-embedded human glioma tissue using anti-Creatine kinase B chain RabMAb (Cat. #AJ1198a).
  • IF - Creatine Kinase B Antibody AJ1198a
    Immunofluorescent staining of HeLa cells using anti-Creatine kinase B chain RabMAb (Cat. #AJ1198a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P12277
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR3927
Calculated MW 42644 Da
Gene ID 1152
Other Names Creatine kinase B-type, B-CK, Creatine kinase B chain, CKB, CKBB
Target/Specificity A synthetic peptide corresponding to residues near the C-term of human Creatine Kinase B type was used as immunogen.
Dilution WB~~1:5000~20000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCreatine Kinase B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CKB
Synonyms CKBB
Function Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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The creatine kinases are a family of enzymes with a highly conserved protein sequence, which are involved in the maintenance of ATP at sites of cellular work .Three cytoplasmic (non-mitochondrial) isoenzymes of creatine kinase (CK)' are readily identified in human tissue. These isoenzymes are dimeric molecules with two dissociable subunits designated as M- (muscle) or B- (brain) type that can reassociate to form the electrophoretically distinct MM, BB, or MB isotypes (1).The protein encoded by creatine kinase B is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate (2). Creatine kinase isoenzymes are crucial to energy metabolism, particularly in tissues with high energy requirements, such as skeletal muscle, heart, brain and spermatozoa (3).


1. Frederic J. Kaye The Journal of Clinical Investigation, Inc. 79:1412-1420, 1987 2. The NCBI handbook [Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; 2002 Oct. Chapter 17, The Reference Sequence (RefSeq) Project. 3. The UniProt Consortium. The Universal Protein Resource (UniProt) Nucleic Acids Res. 37:D169-D174(2009).

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Cat# AJ1198a
(40 western blots)
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