|Application ||WB, IHC|
|Calculated MW||33777 Da|
|Other Names||Crk-like protein, CRKL|
|Target/Specificity||A synthetic peptide corresponding to residues in the SH2 domain, near the N-term of human CrkL, was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CrkL Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May mediate the transduction of intracellular signals.|
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Provided below are standard protocols that you may find useful for product applications.
CrkL (Crk-like), a 36kDa protein predominantly expressed in hematopoietic cell lines, is a member of the Crk adapter protein family. Lacking any intrinsic enzymatic activity, CrkL is composed of one SH2 domain and two SH3 domains (1). The SH2 domain of CrkL as been shown to interact with c-Cbl, Cas, Hef-1 and Paxillin (2); while the N-terminal SH3 can bind to c-ABL, Sos1 and C3G (3). Additionally, the phosphorylated form of CrkL (pY207) binds to BCR-ABL oncogene in chronic myelogenous leukemia cells (4).
1. ten Hoeve J., Morris C., Heisterkamp N., Groffen J.; Oncogene 8:2469-2474(1993).
2. Kyono, W. T., de Jong, R., Park, R. K., Liu, Y., Heisterkamp, N., Groffen, J., and Durden, D. L. (1998) J. Immunol. 161, 5555-5563
3. Sattler, M., Salgia, R., Shrikhande, G., Verma, S., Uemura, N., Law, S. F., Golemis, E. A., and Griffin, J. D. (1997) J. Biol. Chem. 272, 14320-14326
4. Ling, P., Yao, Z., Meyer, C. F., Wang, X. S., Oehrl, W., Feller, S. M., and Tan, T. H. (1999) Mol. Cell. Biol. 19, 1359-1368
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