|Application ||WB, IHC|
|Calculated MW||20159 Da|
|Other Names||Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, CRYAB, CRYA2|
|Target/Specificity||A synthetic peptide corresponding to residues on the C terminus of human Alpha B Crystallin was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Crystallin Alpha B Antibody Crystallin is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.|
|Cellular Location||Cytoplasm. Nucleus. Note=Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles|
|Tissue Location||Lens as well as other tissues.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Alpha B Crystallin, first identified as a structural component of the vertebrate eye lens, is expressed at high levels in lens and at lower levels in a number of other tissues, most notably cardiac and skeletal muscle, kidney, and brain (1). It is a major protein component of Rosenthal fibers, which massively accumulate in the brains of patients suffering from Alexander's disease (2). The major phosphorylation site of Alpha B Crystallin in brains of patients with Alexander's disease or Alzheimer's disease as well as in aged controls is Serine 59 (3). Racemization, isomerization, and oxidation of Alpha B Crystallin are said to occur spontaneously in the aging process (4).
1. Dubin RA, et al. Genomics 7(4):594-601, 1990
2. Iwaki A, et al. Neurosci Lett 140(1):89-92, 1992
3. Kato K, et al. J Neurochem 76(3):730-6, 2001
4. Fujii N, et al. Biochim Biophys Acta 204(2):157-63, 1994
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.