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CUL4A/B Antibody

Rabbit Monoclonal Antibody

  • WB - CUL4A/B Antibody AJ1207b
    A. Western blot analysis on (A) fetal uterus, (B) HeLa, (C) A375 and (D) fetal testis lysates using anti-CUL4A/B RabMAb (Cat. #AJ1207b), dilution 1:5000.
  • IHC - CUL4A/B Antibody AJ1207b
    B. Immunohistochemical staining of paraffin-embedded brain tissue using anti-CUL4A/B RabMAb (Cat. #AJ1207b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13620
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR3200
Calculated MW 103982 Da
Gene ID 8450
Other Names Cullin-4B, CUL-4B, CUL4B, KIAA0695
Target/Specificity A synthetic peptide corresponding to residues in human CUL4B was used as an immunogen.
Dilution WB~~1:2000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCUL4A/B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CUL4B
Synonyms KIAA0695
Function Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation- induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8.
Cellular Location Nucleus
Research Areas
Citations (0)

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Cullin proteins assemble a large number of RING E3 ubiquitin ligases and regulate various physiological processes, including regulating replication and transcription (1-2). Two cullin 4 (CUL4) proteins, CUL4A and CUL4B, have been identified. CUL4A and CUL4B are two closely related proteins, coded by two different genes. The amino acid sequences of CUL4A and CUL4B show 80% similarity, differing by the unique and extended N-terminus of CUL4B. CUL4B is reported to be found in the nucleus as well as the cytoplasm during differentiation, while CUL4A has been reported to be present predominantly in the cytoplasm. CUL4B is suggested to have a more specific role than CUL4A due to the presence of functional nuclear localization signal sequence in the N-terminus. CUL4A is noted as essential for embryonic development (3).


1. Liu J, et al. Mol Cell. 10(6):1511-8, 2002
2. Higa LA, et al. Cell Cycle 5(1):71-7, 2006
3. Shiyanov P, et al. J Biol Chem. 274(50):35309-12, 1999

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Cat# AJ1207b
(40 western blots)
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