|Application ||WB, IHC, IF|
|Calculated MW||33729 Da|
|Other Names||G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene, CCND1, BCL1, PRAD1|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus of human Cyclin D1 was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Cyclin D1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.|
|Cellular Location||Nucleus. Cytoplasm. Membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members|
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Provided below are standard protocols that you may find useful for product applications.
Cyclin D1 belongs to the Cyclin D family. Cyclin D1 is required for the cell cycle G1/S transition (1). Cyclin D1 forms a serine/threonine kinase holoenzyme complex with and functions as a regulatory subunit of CDK4 or CDK6. Cyclin D1 also phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1/S phase (2-3). Amplification or overexpression of cyclin D1 plays a pivotal role in the development of various human cancers including parathyroid adenoma, breast cancer, colon cancer, lymphoma, melanoma, and prostate cancer (4-5). Over-expression of cyclin D1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis (6). Cyclin D1 also associates with, and regulates the activity of transcription factors, coactivators and corepressors that govern histone acetylation and chromatin remodeling proteins (7-8).
1. Inaba et al. Genomics 13: 565-574, 1992.
2. Sherr CJ 2000 The Pezcoller Lecture: Canc Res 60:3689
3. Baldin et al. 1993 Genes Dev 7:812
4. Wang et al. Carcinogenesis 23:257
5. Bala S, Peltomaki P 2001 Cancer Res 61:6042
6. Motokura T, Arnold A 1993 Genes Chrom. Canc. 7:89
7. Wang et al. 2004 Cancer Treat Res 119:217
8. Neumeister et al. Mol Biol Cell 14:2005
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