|Application ||WB, IF|
|Calculated MW||47077 Da|
|Other Names||G1/S-specific cyclin-E1, CCNE1, CCNE|
|Target/Specificity||A synthetic peptide corresponding to residues in human Cyclin E1 was used as immunogen. This antibody is predicted to detect the splice isoform 2 based on sequence analysis.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CCNE1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential for the control of the cell cycle at the G1/S (start) transition.|
|Tissue Location||Highly expressed in testis and placenta. Low levels in bronchial epithelial cells|
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Provided below are standard protocols that you may find useful for product applications.
Cyclin E1 is a member of the cyclin E family that can associate with and activate cyclin-dependent kinase Cdk2. Expression of cyclin E1 is essential for the control of the cell cycle at the late G1 and early S phase (1). The cyclin E/Cdk2 complex plays an essential and limiting role in the transition between G1 and S phase as well as the initiation of DNA replication (2) Ubiquination by the Cul-3 pathway and by Fbw7 regulates cyclin E1 levels and is critically important in normal cells. Abnormally high levels of cyclin E expression have frequently been observed in human cancers (3). Moreover, high cyclin E1 levels correlate with increased tumor aggression and poor prognosis (4).
1. Greider, C.W., and Blackburn, E.H. (1985). Cell 43, 405-413
2. Ohtsubo, M., A. M. Theodoras, J. Schumacher, J. M. Roberts, and M. Pagano. 1995. Mol. Cell. Biol. 15:2612-2624
3. Greider, C.W. (1996). Annu. Rev. Biochem. 65, 337-365
4. Porter et al. 1997. Nat. Med. 3, pp. 222
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