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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   CCNE2 Antibody   

CCNE2 Antibody

Rabbit Monoclonal Antibody

     
  • WB - CCNE2 Antibody AJ1212a
    A. Western blot analysis on Jurkat celll lysate of using anti-Cyclin E2 Rabbit Polyclonal (Cat. #AJ1212a), dilution 1:2000
  • IHC - CCNE2 Antibody AJ1212a
    B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Cyclin-E2 RabMAb. (Cat. #AJ1212a)
  • IF - CCNE2 Antibody AJ1212a
    C. Immunofluorescent staining of HeLa cells using anti-Cyclin-E2 RabMAb. (Cat. #AJ1212a)
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession O96020
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP454Y
Calculated MW 46757 Da
Gene ID 9134
Other Names G1/S-specific cyclin-E2, CCNE2
Target/Specificity A synthetic peptide corresponding to a residue of human Cyclin E2 was used as an immunogen.
Dilution WB~~1:2000~10000
IHC~~1:250~500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCCNE2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCNE2
Function Essential for the control of the cell cycle at the late G1 and early S phase.
Cellular Location Nucleus.
Tissue Location According to PubMed:9858585, highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor- derived cells compared to non-transformed proliferating cells According to PubMed:9840927: low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung According to PubMed:9840943 highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon
Research Areas
Citations (0)

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Background

Cyclin E2 is a member of the cyclin E family that can associate with and activate Cdk2. Expression of cyclin E2 is essential for the control of the cell cycle at the late G1 and early S phase, and this protein may regulate distinct rate-limiting pathway(s) during this phase (1). Cyclin E2 specifically interacts with Cdk inhibitors of the CIP/KIP family and activates both Cdk2 and Cdk3. Expression of the viral E6 oncoprotein in normal human fibroblasts increases levels of cyclin E2, but not cyclin E1, while expression of the E7 oncoprotein upregulates both (2). Abnormally high levels of cyclin E expression have frequently been observed in human cancers (3).

References

1. Lauper, N., et al. Oncogene 17: 2637
2. Zariwala, M., et al. Oncogene 17: 2787
3. Gudas, J.M., et al. Mol Cell Biol. 19: 612

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Discontinued
Cat# AJ1212a
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