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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   Cyclin-A2 Antibody (C-term)   

Cyclin-A2 Antibody (C-term)

Rabbit Monoclonal Antibody

     
  • WB - Cyclin-A2 Antibody (C-term) AJ1214b
    A. Western blot analysis on Hela cell lysate using anti-Cyclin A (C-term) RabMAb (Cat. #AJ1214b), dilution 1:1,000.
  • IHC - Cyclin-A2 Antibody (C-term) AJ1214b
    B. Immunohistochemical analysis of paraffin-embedded skin carcinoma using anti-Cyclin A (C-term) RabMAb (Cat. #AJ1214b).
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P20248
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names Y193
Calculated MW 48551 Da
Gene ID 890
Other Names Cyclin-A2, Cyclin-A, CCNA2, CCN1, CCNA
Target/Specificity A synthetic peptide corresponding to residues near the N-term of human Cyclin A2 was used as immunogen.
Dilution WB~~1:1000~10000
IHC~~1:50~100
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCyclin-A2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCNA2 (HGNC:1578)
Function Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.
Cellular Location Nucleus. Cytoplasm Note=Exclusively nuclear during interphase (PubMed:1312467) Detected in the nucleus and the cytoplasm at prophase (PubMed:1312467). Cytoplasmic when associated with SCAPER (PubMed:17698606).
Research Areas
Citations (0)

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Background

Cyclin A is a member of the Cyclin A family that can bind and activate Cdc2 and Cdk2 and is involved in the control of mitosis and S phase (1). During S phase, cyclin A forms a complex with Cdk2, the pRB-related protein p107 and E2F (2). Additionally, deregulation of cyclin A expression might contribute to tumor formation (3). The cyclin A family consist of two members, cyclin A1 and cyclin A2, to which cyclin A2 promotes both G1/S and G2/M transition (4).

References

1. Pines, J. & Hunter, T. (1990) Nature ( London) 346, 760-762.
2. Devoto, S. H., Mudryj, M., Pines, J., Hunter, T. & Nevins, J. R. (1992) Cell 68, 167-176.
3. Wang, J., Zindy, F., Chenivesse, X., Lamas, E., Henglein, B. & Brechot, C. (1992) Oncogene 7, 1653-1656.
4. Pagano, M.; Pepperkok, R.; Verde, F.; Ansorge, W.; Draetta, G. EMBO J. 11: 961-971, 1992.

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Discontinued
Cat# AJ1214b
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