|Application ||WB, IHC|
|Calculated MW||48551 Da|
|Other Names||Cyclin-A2, Cyclin-A, CCNA2, CCN1, CCNA|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-term of human Cyclin A2 was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Cyclin-A2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.|
|Cellular Location||Nucleus. Cytoplasm Note=Exclusively nuclear during interphase (PubMed:1312467) Detected in the nucleus and the cytoplasm at prophase (PubMed:1312467). Cytoplasmic when associated with SCAPER (PubMed:17698606).|
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Provided below are standard protocols that you may find useful for product applications.
Cyclin A is a member of the Cyclin A family that can bind and activate Cdc2 and Cdk2 and is involved in the control of mitosis and S phase (1). During S phase, cyclin A forms a complex with Cdk2, the pRB-related protein p107 and E2F (2). Additionally, deregulation of cyclin A expression might contribute to tumor formation (3). The cyclin A family consist of two members, cyclin A1 and cyclin A2, to which cyclin A2 promotes both G1/S and G2/M transition (4).
1. Pines, J. & Hunter, T. (1990) Nature ( London) 346, 760-762.
2. Devoto, S. H., Mudryj, M., Pines, J., Hunter, T. & Nevins, J. R. (1992) Cell 68, 167-176.
3. Wang, J., Zindy, F., Chenivesse, X., Lamas, E., Henglein, B. & Brechot, C. (1992) Oncogene 7, 1653-1656.
4. Pagano, M.; Pepperkok, R.; Verde, F.; Ansorge, W.; Draetta, G. EMBO J. 11: 961-971, 1992.
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