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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Cytokeratin-14 Antibody   

Cytokeratin-14 Antibody

Rabbit Monoclonal Antibody

  • WB - Cytokeratin-14 Antibody AJ1219a
    A. Western blot analysis on A431 cell lysate using anti-CK-14 RabMAb (Cat. #AJ1219a), dilution 1:20,000
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P02533
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP1612Y
Calculated MW 51561 Da
Gene ID 3861
Other Names Keratin, type I cytoskeletal 14, Cytokeratin-14, CK-14, Keratin-14, K14, KRT14
Target/Specificity A synthetic peptide corresponding to residues near the C-terminus of human Cytokeratin 14 was used as an immunogen.
Dilution WB~~1:20000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCytokeratin-14 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT14
Function The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Cellular Location Cytoplasm. Nucleus. Note=Expressed in both as a filamentous pattern
Tissue Location Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
Research Areas
Citations (0)

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Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. Cytokeratin 14 (CK-14) is a 50-kDa keratin expressed in abundance in human epidermal cells (1). During anagen, cell proliferation in the germinative matrix of the hair follicle gives rise to the fiber and inner root sheath. The transition from anagen to telogen is marked by downregulation of hair cortical specific keratins and the appearance of CK-14 in the epithelial sac to which the telogen hair fiber is anchored (2). A family with recessive epidermolysis bullosa simplex lacked expression of the basal cell keratin 14. The patients had severe generalized skin blistering that improved slightly with age. The basal cells of the patients did not express keratin 14 and contained no keratin intermediate filaments. The expression of keratin 5, the obligate copolymer of keratin 14, was slightly reduced (3).


1. Marchuk D, et al. Proc Natl Acad Sci 82(6):1609-13, 1985
2. Bowden PE, et al. J Invest Dermatol 110(2):158-64, 1998
3. Jonkman MF, et al. J Invest Dermatol 107(5):764-9, 1996

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Cat# AJ1219a
(40 western blots)
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