|Application ||WB, IHC, IF|
|Calculated MW||49212 Da|
|Other Names||Keratin, type I cytoskeletal 15, Cytokeratin-15, CK-15, Keratin-15, K15, KRT15, KRTB|
|Target/Specificity||A synthetic peptide corresponding to residues on the C-terminus of human CK-15 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Cytokeratin-15 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Tissue Location||Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate|
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Provided below are standard protocols that you may find useful for product applications.
A number of human cytokeratins are expressed during the development of stratified epithelia from one-layered polar epithelia and continue to be expressed in several adult epithelial tissues (1). Cytokeratin 15 (CK15) is a type I keratin without a defined type II partner whose expression in epidermal diseases has not been investigated. A monoclonal antibody raised against the last 17 amino acids of the CK15 polypeptide show that CK-15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Although CK-15 in normal hair follicles was virtually absent from hair bulbs, it was expressed by a subset of keratinocytes in the outer root sheath (2). In human conjunctival epithelium, strong expression of CK-15 was observed in basal cells, whereas CK19 was expressed in both basal and suprabasal layers. Although the expression of K15 and K19 differ in humans and mice, specific staining patterns can be used to characterize the epithelial phenotype in normal and diseased ocular surface (3).
1. Leube RE, et al. J Cell Biol 106(4):1249-61, 1988.
2. Waseem A, et al. J Invest Dermatol 12(3):362-9, 1999
3. Yoshida S, et al. Invest Ophthalmol Vis Sci 47(11):4780-6 2006
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