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Cytokeratin-15 Antibody (N-term)

Rabbit Monoclonal Antibody

  • WB - Cytokeratin-15 Antibody (N-term) AJ1220c
    A. Western blot analysis on A431 cell lysate using anti-Cytokeratin 15 (N-term) RabMAb (Cat. #AJ1220c), dilution 1:5000.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P19012
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP1613
Calculated MW 49212 Da
Gene ID 3866
Other Names Keratin, type I cytoskeletal 15, Cytokeratin-15, CK-15, Keratin-15, K15, KRT15, KRTB
Target/Specificity A synthetic peptide corresponding to residues on the N-terminus of human Cytokeratin 15 was used as an immunogen.
Dilution WB~~1:500~1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCytokeratin-15 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT15
Synonyms KRTB
Tissue Location Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate
Research Areas
Citations (0)

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Cytokeratin 15 (CK15) is a type I keratin without a defined type II partner whose expression in epidermal diseases has not been investigated. A monoclonal antibody raised against the last 17 amino acids of the CK15 polypeptide show that CK-15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Although CK-15 in normal hair follicles was virtually absent from hair bulbs, it was expressed by a subset of keratinocytes in the outer root sheath (2). In human conjunctival epithelium, strong expression of CK-15 was observed in basal cells, whereas CK19 was expressed in both basal and suprabasal layers. Although the expression of K15 and K19 differ in humans and mice, specific staining patterns can be used to characterize the epithelial phenotype in normal and diseased ocular surface (3).


1. Waseem A, et al. J Invest Dermatol 12(3):362-9, 1999 2. Yoshida S, et al. Invest Ophthalmol Vis Sci 47(11):4780-6, 2006

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Cat# AJ1220c
(40 western blots)
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