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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Cytokeratin-20 Antibody   

Cytokeratin-20 Antibody

Rabbit Monoclonal Antibody

  • WB - Cytokeratin-20 Antibody AJ1225a
    A. Western blot analysis on human small intestine lysate using anti-CK20 RabMAb (Cat. #AJ1225a), dilution 1:50,000.
  • IHC - Cytokeratin-20 Antibody AJ1225a
    B. Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-CK-20 RabMAb (Cat. #AJ1225a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35900
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR1622Y
Calculated MW 48487 Da
Gene ID 54474
Other Names Keratin, type I cytoskeletal 20, Cytokeratin-20, CK-20, Keratin-20, K20, Protein IT, KRT20
Target/Specificity A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 20 was used as an immunogen.
Dilution WB~~1:10000~50000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCytokeratin-20 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT20
Function Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
Cellular Location Cytoplasm
Tissue Location Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
Research Areas
Citations (0)

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Intermediate-sized filament (IF) protein designated cytokeratin (CK) 20 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).


1. Moll R, et al. Differentiation 53(2):75-93, 1993
2. Calnek D, et al. Differentiation 53(2):95-104
3. Su YC, et al. Kaohsiung J Med Sci 22(1):14-9, 2006

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Cat# AJ1225a
(40 western blots)
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