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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Cytokeratin-4 Antibody   

Cytokeratin-4 Antibody

Rabbit Monoclonal IgG

     
  • WB - Cytokeratin-4 Antibody AJ1226c
    Western blot analysis on (A) A431 and (B) human uterus cell lysates using anti-Cytokeratin 4 RabMAb (Cat. #AJ1226c).
  • IHC - Cytokeratin-4 Antibody AJ1226c
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 4 RabMAb. Note positive staining of squamous cells (Cat. #AJ1226c).
  • IF - Cytokeratin-4 Antibody AJ1226c
    Immunofluorescent staining of A431 cells using anti- Cytokeratin 4 RabMAb (Cat. #AJ1226c).
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession P19013
Reactivity Human, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR1598
Calculated MW 57285 Da
Other Names Keratin, type II cytoskeletal 4, Cytokeratin-4, CK-4, Keratin-4, K4, Type-II keratin Kb4, KRT4, CYK4
Target/Specificity A synthetic peptide corresponding to residues in human Cytokeratin 4 was used as an immunogen.
Dilution WB~~1:1000~10000
IHC~~1:100~250
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCytokeratin-4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT4
Synonyms CYK4
Tissue Location Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer
Research Areas
Citations (0)

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Background

Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells (1). Keratins are expressed in pairs by epithelial cells in a tissue and cell specific manner (2). The major differentiation specific keratins of the buccal mucosa, nasal, esophageal and anogenital epithelia are cytokeratin 4 and cytokeratin 13 (2). Cytokeratin 4 and cytokeratin 13 form heterodimers in normal epithelial cells (3). In humans, cytokeratin 4 is present in all layers of the epidermis at 10 weeks and gradually disappears (3). Genetic mutations in cytokeratin 4 cause white sponge nevus (WSN), an autosomal dominant disorder that affects noncornified stratified squamous epithelia (4).

References

1. Online Mendelian Inheritance in Man, OMIM (TM). Johns Hopkins University, Baltimore, MD. MIM Number: {123940}: {9/4/2003}: . WWW URL: http://www.ncbi.nlm.nih.gov/omim/ 2. Rugg et al. Nat Genet. 11(4):450-2, 1995. 3. Ness et al. J Biol Chem. 273(37):23904-11, 1998. 4. Terrinoni et al. J Invest Dermatol. 114(2):388-91, 2000.

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Discontinued
Cat# AJ1226c
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