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Cytokeratin-5 Antibody

Rabbit Monoclonal Antibody

     
  • WB - Cytokeratin-5 Antibody AJ1227b
    A. Western blot analysis on A431 cell lysate using anti-CK-5 RabMAb (Cat. #AJ1227b), dilution 1:10,000.
  • IHC - Cytokeratin-5 Antibody AJ1227b
    B. Immunohistochemical analysis of paraffin-embedded human squamous cervical carcinoma using anti-CK-5 RabMAb (Cat. #AJ1227b).
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P13647
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR1600Y
Calculated MW 62378 Da
Gene ID 3852
Other Names Keratin, type II cytoskeletal 5, 58 kDa cytokeratin, Cytokeratin-5, CK-5, Keratin-5, K5, Type-II keratin Kb5, KRT5
Target/Specificity A synthetic peptide corresponding to residues in the head region of human cytokeratin 5 was used as an immunogen.
Dilution WB~~1:5000
IHC~~1:100
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCytokeratin-5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT5
Research Areas
Citations (0)

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Background

Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. The mitotically active basal layers of most stratified squamous epithelia express 10 to 30% of their total protein as keratin. The two keratins specifically expressed in these cells are the type II keratin CK-5 and its corresponding partner, type I keratin CK-14, both of which are essential for the formation of 8-nm filaments (1). CK-5 and calretinin have been useful in different studies as immunohistochemical markers suggestive of mesothelioma, and their expression is analyzed for the histological differential diagnosis with adenocarcinomas, especially when confronting with metastatic tumors of unknown origin (2). Loss-of-function mutations in the keratin 5 gene (KRT5) affected family members and in six unrelated patients with Dowling-Degos disease (DDD), an autosomal dominant genodermatosis. This suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).

References

1. Lersch R, et al. Mol Cell Biol. 9(9):3685-97, 1989.
2. Cid Mouteira P, et al. Actas Urol Esp 30(4):367-71, 2006.
3. Betz RC, et al. Am J Hum Genet 78(3):510-9, 2006.

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Discontinued
Cat# AJ1227b
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