|Application ||WB, IHC, IF|
|Calculated MW||60045 Da|
|Other Names||Keratin, type II cytoskeletal 6A, Cytokeratin-6A, CK-6A, Cytokeratin-6D, CK-6D, Keratin-6A, K6A, Type-II keratin Kb6, Hom s 5, KRT6A, K6A, KRT6D|
|Target/Specificity||A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 6 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Cytokeratin-6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair.|
|Tissue Location||Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath|
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Provided below are standard protocols that you may find useful for product applications.
The human type II Cytokeratin 6 (CK6; 56 kDa) is expressed in a heterogeneous array of epithelial tissues under normal conditions, but is better known for its strong induction in stratified epithelia that feature an enhanced cell proliferation rate or abnormal differentiation. It has been demonstrated that CK6 isoform, CK6a, is clearly the dominant CK6 isoform in skin tissue samples and cultured epithelial cell lines and that the various isoforms are differentially regulated within and between epithelial tissue types (1). The murine genome is known to have two keratin 6 (CK6) genes, mouse CK6, CK6a and MK6b. These genes display a complex expression pattern with constitutive expression in the epithelia of oral mucosa, hair follicles, and nail beds (2). Pachyonychia congenital (PC) is characterized by hypertrophic nail dystrophy and associated ectodermal features. PC-1 subtype is associated with mutations in keratins 6a or 16, whereas PC-2 subtype is linked to mutations in keratins 6b or 17 (3).
1. Takahashi K, et al. J Biol Chem 270(31):18581-92, 1995.
2. Wojcik SM, et al J Cell Biol 154(3):619-30, 2001.
3. Ward KM, et al. Clin Exp Dermatol 28(4):434-6, 2003.
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