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Rabbit Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC |
|---|---|
| Primary Accession | P05787 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone Names | E432 |
| Calculated MW | 53704 Da |
| Gene ID | 3856 |
| Other Names | Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8, KRT8, CYK8 |
| Target/Specificity | A synthetic peptide corresponding to residues surrounding Ser73 of human Cytokeratin 8 was used as immunogen. |
| Dilution | WB~~1:1000~10000 IHC~~1:50~1:100 |
| Format | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Cytokeratin-8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | KRT8 |
|---|---|
| Synonyms | CYK8 |
| Function | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. |
| Cellular Location | Cytoplasm. Nucleus, nucleoplasm Nucleus matrix |
| Tissue Location | Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Keratin polypeptides 8 and 18 (K8/18) are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (1-3). Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate epithelial cells, forming alpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nm diameter filaments (4).
References
1. Ku, N.O. and M.B. Omary. Identification and mutational analysis of the glycosylation sites of human keratin 18. J Biol Chem. 270: 11820
2. Kulesh, D.A. and R.G. Oshima. Cloning of the human keratin 18 gene and its expression in nonepithelial mouse cells. Mol Cell Biol. 8: 1540
3. Marceau, N. and A. Loranger. Cytokeratin expression, fibrillar organization and subtle function in liver cells. Biochem. Cell Biol. 73: 619
4. Fuchs, E. Keratins and the skin. Annu. Rev. Cell. Dev. Biol. 11: 123
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