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Cytokeratin-8 Antibody Phospho (pS73)

Rabbit Monoclonal Antibody

  • WB - Cytokeratin-8 Antibody Phospho (pS73) AJ1230f
    A. Western blot analysis on A431 cell lysates using anti-Phospho-Keratin 8 (pS73) RabMAb (Cat. #AJ1230f), dilution 1:10,000. Cell lysates were either (1) untreated or (2) treated with EGF.
  • IHC - Cytokeratin-8 Antibody Phospho (pS73) AJ1230f
    B. Immunohistochemical analysis of paraffin-embedded liver carcinoma using anti-Phospho-Keratin 8 (pS73) RabMAb (Cat. #AJ1230f).
  • IF - Cytokeratin-8 Antibody Phospho (pS73) AJ1230f
    C. Immunofluorescent staining of A431 cells using anti-Phospho-Keratin 8 (pS73) RabMAb (Cat. #AJ1230f).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05787
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names E431-2
Calculated MW 53704 Da
Gene ID 3856
Other Names Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8, KRT8, CYK8
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Ser73 of human Cytokeratin 8 was used as immunogen. The antibody only detects Keratin 8 phosphorylated on Serine 73.
Dilution WB~~1:5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCytokeratin-8 Antibody Phospho (pS73) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT8
Synonyms CYK8
Function Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Cellular Location Cytoplasm. Nucleus, nucleoplasm Nucleus matrix
Tissue Location Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Research Areas
Citations (0)

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Keratin polypeptides 8 and 18 (K8/18) are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (1-3). Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate epithelial cells, forming alpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nm diameter filaments (4).


1. Ku, N.O. and M.B. Omary. Identification and mutational analysis of the glycosylation sites of human keratin 18. J Biol Chem. 270: 11820
2. Kulesh, D.A. and R.G. Oshima. Cloning of the human keratin 18 gene and its expression in nonepithelial mouse cells. Mol Cell Biol. 8: 1540
3. Marceau, N. and A. Loranger. Cytokeratin expression, fibrillar organization and subtle function in liver cells. Biochem. Cell Biol. 73: 619
4. Fuchs, E. Keratins and the skin. Annu. Rev. Cell. Dev. Biol. 11: 123

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Cat# AJ1230f
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