|Application ||WB, IHC, IF|
|Calculated MW||11165 Da|
|Other Names||Death-associated protein 1, DAP-1, DAP, DAP1|
|Target/Specificity||A synthetic peptide corresponding to C-terminus of human DAP-1 was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DAP-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Negative regulator of autophagy. Involved in mediating interferon-gamma-induced cell death.|
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Provided below are standard protocols that you may find useful for product applications.
Death associated protein 1 (DAP-1) is a 15-kDa protein that functions as a positive mediator of cell death initiated by interferon (IFN-) gamma (1,2). DAP-1 is proline rich and possesses one SH3 binding motif, as well as several consensus protein kinase phosphorylation sites (1). It is localized to the cytoplasm, but the detailed mechanism of its pro-apoptotic function is unclear. DAP-1 and DAP kinase, a unique, 160-kDa calmodulin-dependent serine/threonine kinase, are both unique candidates for positive mediators of programmed cell death induced by IFN-gamma (3).
1. Deiss, L.P., et al. Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death. Genes Dev. 9: 15
2. Levy-Strumpf, N. and A. Kimchi. Death associated proteins (DAPs): from gene identification to the analysis of their apoptotic and tumor suppressive functions. Oncogene 17: 3331
3. Cohen, O. and A. Kimchi. DAP-kinase: from functional gene cloning to establishment of its role in apoptosis and cancer. Cell Death Differ. 8: 6
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