|Calculated MW||52536 Da|
|Other Names||Death-associated protein kinase 3, DAP kinase 3, DAP-like kinase, Dlk, MYPT1 kinase, Zipper-interacting protein kinase, ZIP-kinase, DAPK3, ZIPK|
|Target/Specificity||A synthetic peptide corresponding to residues on the C-terminus of human DAPK3 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DAPK3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase- dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor.|
|Cellular Location||Nucleus Cytoplasm. Note=Predominantly localizes to the cytoplasm but can shuttle between the nucleus and cytoplasm; cytoplasmic localization is promoted by phosphorylation at Thr-299 and involves Rho/Rock signaling. Isoform 2: Nucleus. Cytoplasm|
|Tissue Location||Widely expressed. Isoform 1 and isoform 2 are expressed in the bladder smooth muscle|
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Provided below are standard protocols that you may find useful for product applications.
Death-associated protein kinase (DAPK3), which is a mediator of apoptosis, is induced by gamma interferon. DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities (1). Results suggest that DAPK3, in collaboration with Daxx and Par-4, mediates a novel nuclear pathway for apoptosis (2). It has been shown that the nuclear serine/threonine-specific kinase phosphorylates core histones H3 and H4, and myosine light chain in vitro. HeLa ZIPK phosphorylated the regulatory light chain of myosin II (MRLC) at both serine 19 and threonine 18 in a Ca2+/calmodulin independent manner. It interacts with transcription and splicing factors as well as with pro-apoptotic protein Par-4 suggesting that it participates in multiple cellular processes (3).
1. Kawai T, et al. Mol Cell Biol 18(3):1642-51, 1998
2. Kawai T, et al. Mol Cell Biol 23(17):6174-86, 2003
3. Preuss U, et al. Nucleic Acids Res 31(3):878-85, 2003
4. Murata-Hori M, et al. FEBS Lett 451(1):81-4, 1999
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