|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||22963 Da|
|Other Names||Protein phosphatase 1 regulatory subunit 1B, DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, PPP1R1B, DARPP32|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-term of human DARPP-32 was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DARPP-32 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibitor of protein-phosphatase 1.|
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Provided below are standard protocols that you may find useful for product applications.
The 32kDa dopamine and cyclic adenosine 3',5'-monophosphate-regulated phosphoprotein (DARPP-32) can either function as a serine/threonine kinase or as a serine/threonine phosphatase depending upon which particular amino acid residue is phosphorylated (1). When phosphorylated by protein kinase A (PKA) at threonine 34, DARPP-32 becomes a potent inhibitor of protein phosphatase-1 (2-3). Alternatively, when phosphorylated at threonine 75 by cyclin-dependent kinase 5(Cdk5), DARPP-32 is converted into an inhibitor of PKA (1). DARPP-32 and truncated DARPP (t-DARPP) are overexpressed in gastric cancers (4).
1. Bibb et al. Nature 402: 669-671, 1999.
2. Greengard, P.; Allen, P. B.; Nairn, A. C. : Neuron 23: 435-447, 1999.
3. Hemmings, H. C., Jr.; Greengard, P.; Tung, H. Y.; Cohen, P. : Nature 310: 503-505, 1984.
4. El-Rifai, W.; Smith, M. F., Jr.; Li, G.; Beckler, A.; Carl, V. S.; Montgomery, E.; Knuutila, S.; Moskaluk, C. A.; Frierson, H. F., Jr.; Powell, S. M. :Cancer Res. 62: 4061-4064, 2002.
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