|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||53536 Da|
|Other Names||Desmin, DES|
|Target/Specificity||A synthetic peptide corresponding to C-terminus of human Desmin was used as immunogen. Predicted to cross-react with rat, bovine and pig, based on sequence homology.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Desmin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed:24200904, PubMed:25394388, PubMed:26724190). May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction.|
|Cellular Location||Cytoplasm, myofibril, sarcomere, Z line Cytoplasm. Cell membrane, sarcolemma. Note=Localizes in the intercalated disks which occur at the Z line of cardiomyocytes (PubMed:24200904, PubMed:26724190).|
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Provided below are standard protocols that you may find useful for product applications.
Desmin are class-III intermediate filaments found in muscle cells, and are expressed during the formation of muscle cells or fibers. Cytoskeletal intermediate filaments (IFs) are constructed from two-chain alpha-helical coiled-coil molecules arranged on an imperfect helical lattice (1,2). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. Desmin mutations have been linked to familial cardiac and skeletal myopathy (3,4).
1. Stewart, M. Intermediate filament structure and assembly. Curr. Opin. Cell Biol. 5: 3
2. Li, Z.L., et al. Human desmin-coding gene: complete nucleotide sequence, characterization and regulation of expression during myogenesis and development. Gene 78: 243
3. Li, Z.L. and D. Paulin. High level desmin expression depends on a muscle-specific enhancer. J Biol Chem. 266: 6562
4. Goldfarb, L.G., et al. Missense mutations in desmin associated with familial cardiac and skeletal myopathy. Nat Genet. 19: 402
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