|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||10366 Da|
|Other Names||Dynein light chain 1, cytoplasmic, 8 kDa dynein light chain, DLC8, Dynein light chain LC8-type 1, Protein inhibitor of neuronal nitric oxide synthase, PIN, DYNLL1, DLC1, DNCL1, DNCLC1, HDLC1|
|Target/Specificity||A synthetic peptide corresponding to residues on the N-terminus of human DLC8 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DLC8 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||DLC1, DNCL1, DNCLC1, HDLC1|
|Function||Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures. Promotes transactivation functions of ESR1 and plays a role in the nuclear localization of ESR1.|
|Cellular Location||Cytoplasm, cytoskeleton. Nucleus. Mitochondrion. Note=Upon induction of apoptosis translocates together with BCL2L11 to mitochondria|
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DLC8 protein bound efficiently to gephyrin in in vitro binding assays and colocalized with gephyrin during coexpression in HEK293 cells. The binding site for DLC8 was mapped to a fragment of 63 amino acids within the central linker domain of gephyrin. In hippocampal neurons, endogenous DLC8 protein was enriched at synaptic sites identified by synaptophysin and gephyrin immunostaining. Because DLC8 has been described as stoichiometric components of cytoplasmic dynein and myosin-Va complexes, results suggest that motor proteins are involved in the subcellular localization of gephyrin (1). DLC8 was identified as a transport molecule in the cytoplasm. DLC8 co-localizes with TRPS1 in dot-like structures in the cell nucleus. In an electrophoretic mobility shift assay it was shown that the interaction of DLC8 and TRPS1 lowers the binding of TRPS1 to the GATA consensus sequence. In addition DLC8 is able to suppress the transcriptional repression activity of TRPS1 (2).
1. Fuhrmann JC, et al. J Neurosci 22(13):5393-402, 2002.
2. Kaiser FJ, et al. Hum Mol Genet 12(11):1349-58, 2003.
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