- CITATIONS: 1
|Application ||WB, IHC|
|Calculated MW||97456 Da|
|Other Names||Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324, E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3, CDH1, CDHE, UVO|
|Target/Specificity||A synthetic peptide corresponding to residues in the 5th cadherin domain of human E-cadherin was used.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||E-Cadherin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.|
|Cellular Location||Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma- catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane|
|Tissue Location||Non-neural epithelial tissues.|
Provided below are standard protocols that you may find useful for product applications.
E-Cadherin, a member of the cadherin superfamily, is implicated as a key player in different cellular processes including development, morphology, polarity, migration and tissue integrity (1-2). E-cadherin is a glycoprotein with an extracellular domain that interacts with other E-cadherin molecules on adjacent cells, thereby establishing adhesion between epithelial cells. Established as a tumor and metastasis suppressor, E-cadherin expression is frequently down-regulated or extinguished in malignancy which strongly correlates with poor prognosis (3-4). Mutations in E-cadherin are found in gastric, breast, colorectal, thyroid and ovarian cancer (5).
1. Mansouri, A.; Goodfellow, P. N.; Kemler, R. : Cytogenet. Cell Genet. 46: 655, 1987.
2. Vleminckx K, Kemler R. Bioessays. 1999;21:211
3. Nollet, F., G. Berx, and F. van Roy. 1999. Mol. Cell Biol. Res. Comm. 2:77
4. Birchmeier, W., and J. Behrens. 1994. Biochim. Biophys. Acta. 1198:11
5. Thoreson MA, Reynolds AB. Differentiation. 2002 Dec;70(9-10):583-9
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