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eEF2 Antibody

Rabbit Monoclonal Antibody

  • WB - eEF2 Antibody AJ1250a
    A. Western blot analysis on293 cell lysate using anti-eEF2 RabMAb (Cat. #AJ1250a), dilution 1:1,000.
  •  - eEF2 Antibody AJ1250a
    B. Immmunohistochemical analysis of paraffin-embedded human lymphoma using anti-eEF2 RabMAb (Cat. #AJ1250a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P13639
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP723Y
Calculated MW 95338 Da
Gene ID 1938
Other Names Elongation factor 2, EF-2, EEF2, EF2
Target/Specificity A synthetic peptide corresponding to residues near the N-terminus of human eEF2 was used as immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionseEF2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EEF2
Synonyms EF2
Function Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Cellular Location Cytoplasm. Nucleus. Note=Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product.
Citations (0)

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The eukaryotic translation Elongation Factor 2 (eEF2) is a 95 kDa member of the G-protein superfamily. Following peptide bond formation, eEF2 catalyzes translocation of the deacylated tRNA in the P-site and peptidyl tRNA in the A-site into the E- and P- sites, respectively (1). The activity of eEF2 is regulated by phosphorylation (2). To be active, eEF2 must be dephosphorylated, and phosphorylation at Thr-56 causes inactivation, resulting in the termination of mRNA translation (3). eEF2 is phosphorylated by a specific, calcium and calmodulin (Ca/CaM)-dependent eEF2 kinase (4).


1. Rapp, G.; Klaudiny, J.; Hagendorff, G.; Luck, M. R.; Scheit, K. H. : Biol. Chem. Hoppe-Seyler 370: 1071-1075, 1989.
2. Carlberg, U., Nilsson, A. and Nygard, O. (1990) Eur. J. Biochem. 191, 639
3. Price, N.T., Redpath, N.T., Severinov, K.V., Campbell, D.G., Russell, J.M. and Proud, C.G. (1991) FEBS Lett. 282, 253
4. Ryazanov, A.G., Natapov, P.G., Shestakova, E.A., Severin, F.F. and Spirin, A.S. (1988) Biochimie 70, 619

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Cat# AJ1250a
(40 western blots)
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