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>   home   >   Products   >   Primary Antibodies   >   eEF2 Antibody (C-term)   

eEF2 Antibody (C-term)

Rabbit Monoclonal Antibody

  • WB - eEF2 Antibody (C-term) AJ1250c
    A. Western blot analysis on (A) 293 (B) HeLa (C) A431 (D) NIH3T3 cell lysate using anti-eEF2 RabMAb (Cat. #AJ1250c)
  • IHC - eEF2 Antibody (C-term) AJ1250c
    B. Immunohistochemical analysis of paraffin-embedded human kidney carcinoma using anti-eEF2 RabMAb (Cat. #AJ1250c).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P13639
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP880Y
Calculated MW 95338 Da
Gene ID 1938
Other Names Elongation factor 2, EF-2, EEF2, EF2
Target/Specificity A synthetic peptide corresponding to residues on the C-terminus of human eEF2 was used as an immunogen.
Dilution WB~~1:1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionseEF2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EEF2
Synonyms EF2
Function Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Cellular Location Cytoplasm. Nucleus. Note=Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product.
Citations (0)

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The eukaryotic translation Elongation Factor 2 (eEF2) is a 95 kDa member of the G-protein superfamily. Following peptide bond formation, eEF2 catalyzes translocation of the deacylated tRNA in the P-site and peptidyl tRNA in the A-site into the E- and P- sites, respectively (1). The activity of eEF2 is regulated by phosphorylation (2). To be active, eEF2 must be dephosphorylated, and phosphorylation at Thr-56 causes inactivation, resulting in the termination of mRNA translation (3). eEF2 is phosphorylated by a specific, calcium and calmodulin (Ca/CaM)-dependent eEF2 kinase (4).


1. Rapp, G. et al, Biol. Chem. 370: 1071-1075, 1989.
2. Carlberg, U, et al. (1990) Eur. J. Biochem. 191, 639
3. Price, N.T, et al. (1991) FEBS Lett. 282, 253
4. Ryazanov, A.G., et al (1988) Biochimie 70, 619

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Cat# AJ1250c
(40 western blots)
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