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eEF-2K AntibodyRabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1251a | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
eEF-2K Antibody - Product info | |
| Application | WB
|
| Primary Accession | O00418 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | EP724Y |
| Calculated MW | 82144 Da |
| Gene ID 29904 | |
| Other Names EEF2K, Elongation factor 2 kinase, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase | |
| Target/Specificity A synthetic peptide corresponding to residues in the Calmodulin binding region of human eEF2 was used as immunogen. | |
| Dilution WB~~1: 2000~5000 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions eEF-2K Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
eEF-2K Antibody - Protein Information | |
| Name EEF2K | |
| Function Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced | |
eEF-2K Antibody - Related products
AP8162a: EEF2K Antibody (N-term)
RI11687: EEF2K predesign siRNA
eEF-2K Antibody - Application data
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A. Western blot analysis on HeLa (lane A) and Jurkat (lane B) using anti- eEF-2K RabMAb (Cat. #AJ1251a).
eEF-2K Antibody - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Eukaryotic elongation factor-2 kinase (eEF-2k) previously known as Ca2+/calmodulin-dependent protein kinase III, is an abundant cytoplasmic protein highly specific for elongation factor-2 (eEf-2) (1). Phosphorylation of eEF-2 by eEF-2 kinase on specific threonine residues results in the inactivation of eEF-2 and in termination of mRNA translation (2). The activity of eEF2 kinase is not only dependent upon Ca2+ ions, calmodulin (CaM) and insulin, but is also regulated both negatively and positively by phosphorylation by different protein kinases (AMPK, S6K1, p90 RSK) (3-4). There is also evidence that eEF-2 phosphorylation is involved in the regulation of cell cycle progression, cellular differentiation, oogensis and malignant tumors (5-6).
REFERENCES
1. Ryazanov et al. Proc Nat Acad Sci 94: 4884-4889, 1997
2. Ryazanov AG, et al. Nature 334:170-173, 1988.
3. Diggle, TA, et al. FEBS Lett 457:189-192, 1999.
4. Wang, XW, EMBO J 20:4370-4379, 2001.
5. End D, et al. J Biol Chem 257:9223-9225, 1988.
6. Severinov, KV, et al. New Biol 2: 887-893, 1990.
