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> home > Products > Antibodies > Biological Process > Protein Amino Acid Phosphorylation > EGFR Antibody

EGFR AntibodyRabbit Monoclonal Antibody

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Catalog #	Size	Availability	Price
AJ1252b	100ul 400 ul	2-3 days	$ 315.00	DISCONTINED INQUIRE CLICK INQUIRE Add to cart

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EGFR Antibody - Product info
Application	WB, IHC Applications Legend: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Primary Accession	P00533
Reactivity	Human, Mouse, Rat
Clone Names	EP38Y
Calculated MW	134277 Da
Gene ID 1956
Other Names EGFR, ERBB1, Epidermal growth factor receptor, Receptor tyrosine-protein kinase ErbB-1
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human EGF receptor was used as immunogen. The antibody detects both EGFR phosphorylated on Tyrosine 1068 of the mature human isoform 1 (corresponding to Y1092 from the precursor form P00533-1/p170), and also unphosphorylated EGFR.
Dilution WB~~1:10000 IHC~~1:100~250
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions EGFR Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
EGFR Antibody - Protein Information
Name EGFR
Synonyms ERBB, ERBB1, HER1
Function Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin
Cellular Location Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand
Tissue Location Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers

EGFR Antibody - Related products

AM7628a: EGFR Antibody (Ascites)

AM7628b: EGFR Antibody

AP3305a: Phospho-EGFR-Y869 Antibody

AP3306a: Phospho-EGFR-S1026 Antibody

AP3307a: Phospho-EGFR-Y1069 Antibody

AP3308a: Phospho-EGFR-S1166 Antibody

AP3348a: Phospho-EGFR-S1190 Antibody

AP3375a: Phospho-EGFR-S695 Antibody

AP3376a: Phospho-EGFR-Y1125 Antibody

AP3384a: Phospho-EGFR-Y998 Antibody

AP3407a: Phospho-EGFR-pY1172 Antibody

AP3411a: Phospho-EGFR-pY1092 Antibody

AP3423a: Phospho-EGFR-pS1070 Antibody

AP3486a: Phospho-EGFR-T678 Antibody

AP3507a: Phospho-EGFR-pY1016 Antibody

AP3510a: Phospho-EGFR-pS768 Antibody

AP5436a: EGFR-S1026 Antibody (C-term)

AP7628a: EGFR (ErbB1) Antibody (N-term)

AP7628b: EGFR (ErbB1) Antibody (C-term)

AP7628f: EGFR Antibody (Center E746)

AP7628g: EGFR Antibody (S695)

AP7628h: EGFR Antibody (Y869)

AP7628i: EGFR Antibody (Y978)

AP7628j: EGFR Antibody (S991)

AP7628k: EGFR Antibody (S1071)

AP7628l: EGFR Antibody (Y1125)

AP7628m: EGFR Antibody (Y1110)

AP7628n: EGFR Antibody (Y1016)

AP7628o: EGFR Antibody (Y1172)

AP7628p: EGFR Antibody (Y1092)

AP7628s: EGFR Antibody (S1070)

AP7628t: EGFR Antibody (Y998)

AP7628u: EGFR-S1026 Antibody

AP7628v: EGFR Antibody

RI11700: EGFR predesign siRNA

BP3305a: Phospho-EGFR-Y869 Antibody Blocking Peptide

BP3306a: Phospho-EGFR-S1026 Antibody Blocking Peptide

BP3307a: Phospho-EGFR-Y1069 Antibody Blocking Peptide

BP3308a: Phospho-EGFR-S1166 Antibody Blocking Peptide

BP3348a: Phospho-EGFR-S1190 Antibody Blocking Peptide

BP3375a: Phospho-EGFR-S695 Antibody Blocking Peptide

BP3376a: Phospho-EGFR-Y1125 Antibody Blocking Peptide

BP3384a: Phospho-EGFR-Y998 Antibody Blocking Peptide

BP3407a: Phospho-EGFR-pY1172 Antibody Blocking Peptide

BP3411a: Phospho-EGFR-pY1092 Antibody Blocking Peptide

BP3423a: Phospho-EGFR-pS1070 Antibody Blocking Peptide

BP3486a: Phospho-EGFR-T678 Antibody Blocking Peptide

BP3507a: Phospho-EGFR-pY1016 Antibody Blocking Peptide

BP3510a: Phospho-EGFR-pS768 Antibody Blocking Peptide

BP7628a: ErbB1 Antibody (N-term) Blocking Peptide

BP7628b: ErbB1 Antibody (C-term) Blocking Peptide

BP7628g: EGFR Antibody (S695) Blocking Peptide

BP7628h: EGFR Antibody (Y869) Blocking Peptide

BP7628i: EGFR Antibody (Y978) Blocking Peptide

BP7628j: EGFR Antibody (S991) Blocking Peptide

BP7628k: EGFR Antibody (S1071) Blocking Peptide

BP7628l: EGFR Antibody (Y1125) Blocking Peptide

BP7628m: EGFR Antibody (Y1110) Blocking Peptide

BP7628n: EGFR Antibody (Y1016) Blocking Peptide

BP7628o: EGFR Antibody (Y1172) Blocking Peptide

BP7628p: EGFR Antibody (Y1092) Blocking Peptide

BP7628s: EGFR Antibody (S1070) Blocking Peptide

BP7628u: EGFR-pS1026 Blocking Peptide

AJ1252a: EGFR Antibody

AJ1252b: EGFR Antibody

AJ1252c: EGFR Antibody

AJ1252d: EGFR Antibody (C-term)

AJ1252e: EGFR Antibody (N-term)

AJ1252f: EGFR Antibody Phospho (pS1070/1071)

AJ1252g: EGFR Antibody Phospho (pS695)

AJ1252h: EGFR Antibody Phospho (pT678)

AJ1252i: EGFR Antibody Phospho (pT693)

AJ1252j: EGFR Antibody Phospho (pY1068)

AJ1252k: EGFR Antibody Phospho (pY1068)

AJ1252l: EGFR Antibody Phospho (pY1086)

AJ1252m: EGFR Antibody Phospho (pY1110)

AJ1252n: EGFR Antibody Phospho (pY1173)

AJ1252o: EGFR Antibody Phospho (pY845)

EGFR Antibody - Application data

A. Western blot analysis on HeLa cell lysate using anti-EGFR RabMAb (Cat. #AJ1252b), dilution 1:100,000
B. Immunohistochemical staining of paraffin-embedded human lymph node metastasis using anti-EGFR RabMAb (Cat. #AJ1252b).

EGFR Antibody - Research Areas

Antibodies Biological Process Protein Amino Acid Phosphorylation Post-translational Protein Modification Phosphate Metabolic Process Phosphorus Metabolic Process Phosphorylation Protein Modification Process Biopolymer Modification Metabolic Process Primary Metabolic Process Protein Metabolic Process Cellular Protein Metabolic Process Cellular Metabolic Process Cellular Process Positive Regulation Of Biological Process Positive Regulation Of Cellular Process Regulation Of Cell Death Regulation Of Programmed Cell Death Response To Organic Substance Regulation Of Apoptosis Positive Regulation Of Cellular Metabolic Process Positive Regulation Of Metabolic Process Regulation Of Phosphorylation Negative Regulation Of Cellular Process Negative Regulation Of Biological Process Response To Chemical Stimulus Regulation Of Phosphate Metabolic Process Regulation Of Phosphorus Metabolic Process Positive Regulation Of Biosynthetic Process Positive Regulation Of Cellular Biosynthetic Process

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BACKGROUND

Epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). The carboxy terminal tyrosine residues on EGFR, Tyr 1068, Tyr 1148, and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding (3). Once activated, EGFR phosphotyrosines Y-1068 and Y-1173 mediate the binding of Grb2 to the EGFR. Addtionally, phosphotyrosine Y-1173 is a major binding site for SHC on the EGFR (4).

REFERENCES

1. Hunter T, et al. Nature 311: 414
2. Reynolds, F.H. Jr., et al. Nature 292: 259
3. Helin, K., et al. Oncogene 6: 825
4. Batzer, A.G., et al. Mol. Cell. Biol. 14: 5192