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EGFR Antibody Phospho (pS1070/1071)

Rabbit Monoclonal Antibody

  • WB - EGFR Antibody Phospho (pS1070/1071) AJ1252f
    A. Western blot analysis on A431 cell lysates using anti-Phospho-EGFR (pS1070/1071) RabMAb (Cat. #AJ1252f), 1:1,000,000 dilution. Cells were either (A) untreated (B) treated with EGF
  • IF - EGFR Antibody Phospho (pS1070/1071) AJ1252f
    B. Immunofluorescence staining of A431 cells using anti-EGFR RabMAb (Cat. #AJ1252f).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00533
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP2259Y
Calculated MW 134277 Da
Gene ID 1956
Other Names Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Serine 1070 and 1071 of human EGF receptor was used as immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEGFR Antibody Phospho (pS1070/1071) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms ERBB, ERBB1, HER1
Function Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Cellular Location Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF)
Tissue Location Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
Research Areas
Citations ( 0 )


Epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that, when activated by epidermal growth factor (EGF), affects cell growth and differentiation in normal and cancer cells (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). The carboxy terminal tyrosine residues on EGFR, Tyr 1068, Tyr 1148, and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding (3). EGFR overexpression is exhibited various cancer, such as glioma, colorectal carcinoma, breast carcinoma, and head and neck carcinoma (4). Phosphorylation at Ser 1070 has been implicated in altered receptor desensitization and receptor internalization modification of EGFR (5).


1. Hunter T. et al. Nature 311: 414
2. Reynolds FH, et al. Nature 292: 259
3. Helin K, et al. Oncogene 6: 825
4. Christensen ME, et al. Danish Med Bull 45(2):121-134, 1998.
5. Countaway JL, et al. JBC 267:1129-1140, 1992

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Cat# AJ1252f
(40 western blots)
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