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> home > Products > Antibodies > Biological Process > Protein Amino Acid Phosphorylation > EGFR Antibody Phospho (pT693)

EGFR Antibody Phospho (pT693)Rabbit Monoclonal Antibody

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Catalog #	Size	Availability	Price
AJ1252i	100ul 400 ul	2-3 days	$ 355.00	DISCONTINED INQUIRE CLICK INQUIRE Add to cart

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EGFR Antibody Phospho (pT693) - Product info
Application	WB Applications Legend: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Primary Accession	P00533
Reactivity	Human
Clone Names	EP2256Y
Calculated MW	134277 Da
Gene ID 1956
Other Names EGFR, ERBB1, Epidermal growth factor receptor, Receptor tyrosine-protein kinase ErbB-1
Target/Specificity A phospho specific peptide corresponding to residues surrounding threonine 693 of human EGFR was used as an immunogen. This antibody detects EGFR phosphorylated on thr693.
Dilution WB~~1:10000~50000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions EGFR Antibody Phospho (pT693) is for research use only and not for use in diagnostic or therapeutic procedures.
EGFR Antibody Phospho (pT693) - Protein Information
Name EGFR
Synonyms ERBB, ERBB1, HER1
Function Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin
Cellular Location Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand
Tissue Location Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers

EGFR Antibody Phospho (pT693) - Related products

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AM7628b: EGFR Antibody

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AP3306a: Phospho-EGFR-S1026 Antibody

AP3307a: Phospho-EGFR-Y1069 Antibody

AP3308a: Phospho-EGFR-S1166 Antibody

AP3348a: Phospho-EGFR-S1190 Antibody

AP3375a: Phospho-EGFR-S695 Antibody

AP3376a: Phospho-EGFR-Y1125 Antibody

AP3384a: Phospho-EGFR-Y998 Antibody

AP3407a: Phospho-EGFR-pY1172 Antibody

AP3411a: Phospho-EGFR-pY1092 Antibody

AP3423a: Phospho-EGFR-pS1070 Antibody

AP3486a: Phospho-EGFR-T678 Antibody

AP3507a: Phospho-EGFR-pY1016 Antibody

AP3510a: Phospho-EGFR-pS768 Antibody

AP5436a: EGFR-S1026 Antibody (C-term)

AP7628a: EGFR (ErbB1) Antibody (N-term)

AP7628b: EGFR (ErbB1) Antibody (C-term)

AP7628f: EGFR Antibody (Center E746)

AP7628g: EGFR Antibody (S695)

AP7628h: EGFR Antibody (Y869)

AP7628i: EGFR Antibody (Y978)

AP7628j: EGFR Antibody (S991)

AP7628k: EGFR Antibody (S1071)

AP7628l: EGFR Antibody (Y1125)

AP7628m: EGFR Antibody (Y1110)

AP7628n: EGFR Antibody (Y1016)

AP7628o: EGFR Antibody (Y1172)

AP7628p: EGFR Antibody (Y1092)

AP7628s: EGFR Antibody (S1070)

AP7628t: EGFR Antibody (Y998)

AP7628u: EGFR-S1026 Antibody

AP7628v: EGFR Antibody

RI11700: EGFR predesign siRNA

BP3305a: Phospho-EGFR-Y869 Antibody Blocking Peptide

BP3306a: Phospho-EGFR-S1026 Antibody Blocking Peptide

BP3307a: Phospho-EGFR-Y1069 Antibody Blocking Peptide

BP3308a: Phospho-EGFR-S1166 Antibody Blocking Peptide

BP3348a: Phospho-EGFR-S1190 Antibody Blocking Peptide

BP3375a: Phospho-EGFR-S695 Antibody Blocking Peptide

BP3376a: Phospho-EGFR-Y1125 Antibody Blocking Peptide

BP3384a: Phospho-EGFR-Y998 Antibody Blocking Peptide

BP3407a: Phospho-EGFR-pY1172 Antibody Blocking Peptide

BP3411a: Phospho-EGFR-pY1092 Antibody Blocking Peptide

BP3423a: Phospho-EGFR-pS1070 Antibody Blocking Peptide

BP3486a: Phospho-EGFR-T678 Antibody Blocking Peptide

BP3507a: Phospho-EGFR-pY1016 Antibody Blocking Peptide

BP3510a: Phospho-EGFR-pS768 Antibody Blocking Peptide

BP7628a: ErbB1 Antibody (N-term) Blocking Peptide

BP7628b: ErbB1 Antibody (C-term) Blocking Peptide

BP7628g: EGFR Antibody (S695) Blocking Peptide

BP7628h: EGFR Antibody (Y869) Blocking Peptide

BP7628i: EGFR Antibody (Y978) Blocking Peptide

BP7628j: EGFR Antibody (S991) Blocking Peptide

BP7628k: EGFR Antibody (S1071) Blocking Peptide

BP7628l: EGFR Antibody (Y1125) Blocking Peptide

BP7628m: EGFR Antibody (Y1110) Blocking Peptide

BP7628n: EGFR Antibody (Y1016) Blocking Peptide

BP7628o: EGFR Antibody (Y1172) Blocking Peptide

BP7628p: EGFR Antibody (Y1092) Blocking Peptide

BP7628s: EGFR Antibody (S1070) Blocking Peptide

BP7628u: EGFR-pS1026 Blocking Peptide

AJ1252a: EGFR Antibody

AJ1252b: EGFR Antibody

AJ1252c: EGFR Antibody

AJ1252d: EGFR Antibody (C-term)

AJ1252e: EGFR Antibody (N-term)

AJ1252f: EGFR Antibody Phospho (pS1070/1071)

AJ1252g: EGFR Antibody Phospho (pS695)

AJ1252h: EGFR Antibody Phospho (pT678)

AJ1252i: EGFR Antibody Phospho (pT693)

AJ1252j: EGFR Antibody Phospho (pY1068)

AJ1252k: EGFR Antibody Phospho (pY1068)

AJ1252l: EGFR Antibody Phospho (pY1086)

AJ1252m: EGFR Antibody Phospho (pY1110)

AJ1252n: EGFR Antibody Phospho (pY1173)

AJ1252o: EGFR Antibody Phospho (pY845)

EGFR Antibody Phospho (pT693) - Application data

A. Western blot analysis on A431 cell lysates using anti-Phospho-EGFR (pT693) RabMAb (Cat. #AJ1252i), 1:20,000 dilution. Cells were either (A) untreated (B) treated with EGF.

EGFR Antibody Phospho (pT693) - Research Areas

Antibodies Biological Process Protein Amino Acid Phosphorylation Post-translational Protein Modification Phosphate Metabolic Process Phosphorus Metabolic Process Phosphorylation Protein Modification Process Biopolymer Modification Metabolic Process Primary Metabolic Process Protein Metabolic Process Cellular Protein Metabolic Process Cellular Metabolic Process Cellular Process Positive Regulation Of Biological Process Positive Regulation Of Cellular Process Regulation Of Cell Death Regulation Of Programmed Cell Death Response To Organic Substance Regulation Of Apoptosis Positive Regulation Of Cellular Metabolic Process Positive Regulation Of Metabolic Process Regulation Of Phosphorylation Negative Regulation Of Cellular Process Negative Regulation Of Biological Process Response To Chemical Stimulus Regulation Of Phosphate Metabolic Process Regulation Of Phosphorus Metabolic Process Positive Regulation Of Biosynthetic Process Positive Regulation Of Cellular Biosynthetic Process

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BACKGROUND

Epidermal growth factor receptor (EGFR) is a 175 kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). The epidermal growth factor (EGF) receptor is regulated by EGF-stimulated autophosphorylation and by phorbol ester-stimulated, protein kinase C (Ca2+/ phospholipid-dependent enzyme) mediated phosphorylation at identified sites. The EGF receptor contains additional phosphorylation sites including a prominent phosphothreonine and several phosphoserines which account for the majority of phosphate covalently bound to the receptor in vivo. Analysis of predicted secondary structure of the EGF receptor reveals that all of the phosphorylation sites are located near beta turns (3).

REFERENCES

1. Hunter, T. et al. Nature 311: 414-416 (1984).
2. Reynolds, F.H. Jr., et al. Nature 292: 259-262 (1981)
3. Heisermann GJ et al. J Biol Chem 263(26):14152-8, 1988.