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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   EGFR Antibody Phospho (pY1068)   

EGFR Antibody Phospho (pY1068)

Rabbit Monoclonal Antibody

  • WB - EGFR Antibody Phospho (pY1068) AJ1252j
    A. Western blot analysis on A431 cell lysate using anti-Phospho-EGFR (pY1068) RabMAb (Cat. #AJ1252j); dilution 1:10,000. Cell lysates were either (1) untreated or (2) treated with EGF.
  • IHC - EGFR Antibody Phospho (pY1068) AJ1252j
    B. Immunohistochemical analysis of paraffin-embedded prostate cancer using anti-Phospho-EGFR (pY1068) RabMAb (Cat. #AJ1252j).
  • IF - EGFR Antibody Phospho (pY1068) AJ1252j
    C. Immunofluorescent staining of A431 cells using anti-Phospho-EGFR (pY1068) RabMAb (Cat. #AJ1252j).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00533
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names Y38
Calculated MW 134277 Da
Gene ID 1956
Other Names Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human EGF receptor was used as immunogen. The antibody only detects EGFR phosphorylated on Tyrosine 1068 of the mature human isoform 1 (corresponding to Y1092 from the precursor form P00533-1/p170).
Dilution WB~~1:20000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEGFR Antibody Phospho (pY1068) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms ERBB, ERBB1, HER1
Function Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Cellular Location Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF)
Tissue Location Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
Research Areas
Citations (0)

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Epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). The carboxy terminal tyrosine residues on EGFR, Tyr 1068, Tyr 1148, and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding (3). Once activated, EGFR phosphotyrosines Y-1068 and Y-1173 mediate the binding of Grb2 to the EGFR. Additionally, phosphotyrosine Y-1173 is a major binding site for SHC on the EGFR (4).


1. Hunter, T. The epidermal growth factor receptor gene and its product. Nature 311: 414
2. Reynolds, F.H. Jr., et al. Human transforming growth factors induces tyrosine phosphorylation of EGF receptors. Nature 292: 259
3. Helin, K., et al. The biological activity of the human epidermal growth factor receptor is positively regulated by its C-terminal tyrosines. Oncogene 6: 825
4. Batzer, A.G., et al. Hierarchy of binding site for Grb2 and Shc on the epidermal growth factor receptor. Mol. Cell. Biol. 14: 5192

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Cat# AJ1252j
(40 western blots)
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