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eIF-2a Antibody Phospho (pS51)

Rabbit Monoclonal Antibody

  • WB - eIF-2a Antibody Phospho (pS51) AJ1253a
    A. Western blot analysis on PC12 cell lysate using anti- Phospho-eIF-2alpha (pS51) RabMAb (Cat. #AJ1253a); dilution 1:1,000. A: non treated cells B: treated with NGF C: treated with Pervanadate D: treated with FBS
  • IHC - eIF-2a Antibody Phospho (pS51) AJ1253a
    B. Immunohistochemical analysis of paraffin-embedded human liver carcinoma using anti-Phospho-eIF-2alpha (pS51) RabMAb (Cat. #AJ1253a).
  • DB - eIF-2a Antibody Phospho (pS51) AJ1253a
    C. Dot Blot analysis using anti-Phospho eIF2alpha (pS51) (Cat. #AJ1253a) against eIF2alpha peptides (A) phosphorylated at Serine 51 and (B) non-phosphorylated peptide.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05198
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names E90
Calculated MW 36112 Da
Gene ID 1965
Other Names Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2S1, EIF2A
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Ser51 of human eIF-2a was used as immunogen. The antibody only detects eIF-2a phosphorylated on Serine 51. Predicted to cross-react with mouse, rat, bovine and pig, based on sequence homology.
Dilution IHC~~1:50~1:100
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionseIF-2a Antibody Phospho (pS51) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF2S1
Synonyms EIF2A
Function Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Cellular Location Cytoplasmic granule. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity).
Research Areas
Citations (0)

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Eukaryotic initiation factor 2 (eIF2) plays a central role in initiating translation, and provides for a rate-limiting step in protein synthesis. Phosphorylation of a-subunit of eIF2 effectively prevents formation of the eIF2/GTP/Met-tRNAi complex and inhibits global protein synthesis (1-3). Three distinct protein kinases regulate protein synthesis in eukaryotic cells by phosphorylating the a-subunit of eIF2 at Serine51 (3). Phosphorylation occurs under a wide variety of different stimuli, including heat shock, serum deprivation, glucose starvation, amino acid starvation, exposure to heavy metal ions, and viral infection (3).


1. Ernst, H., et al. Cloning and sequencing of complementary DNAs encoding the alpha-subunit of translational initiation factor eIF-2. Characterization of the protein and its messenger RNA. J Biol Chem.
2. Kimball, S.R. Eukaryotic initiation factor eIF2. Int. J. Biochem. Cell Biol. 31: 25
3. De Haro, C., et al. The eIF-2alpha kinases and the control of protein synthesis. FASEB J. 10: 1378

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Cat# AJ1253a
(40 western blots)
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