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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   eIF-4B Antibody Phospho (pS504)   

eIF-4B Antibody Phospho (pS504)

Rabbit Monoclonal Antibody

  • WB - eIF-4B Antibody Phospho (pS504) AJ1254b
    A. Western blot analysis on 293T cell lysates using anti-Phospho-eIF4B (pS504) RabMAb (Cat. #AJ1254b), 1:50,000 dilution. Cells were either (A) untreated (B) treated with AP.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P23588
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP2261Y
Calculated MW 69151 Da
Gene ID 1975
Other Names Eukaryotic translation initiation factor 4B, eIF-4B, EIF4B
Target/Specificity A phospho specific peptide corresponding to residues surrounding S504 of human eIF4B was used as an immunogen. This antibody detects eIF4B phosphorylated at serine 504.
Dilution WB~~1:500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionseIF-4B Antibody Phospho (pS504) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF4B
Function Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Research Areas
Citations (0)

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Eukaryotic protein synthesis initiation factor 4B (eIF-4B) is an 80,000 dalton polypeptide which is essential for the binding of mRNA to ribosomes. The amino-terminal domain of eIF-4B contains a consensus RNA binding domain present in a number of other RNA binding proteins (1). It contains a ribonucleoprotein consensus sequence (RNP-CS)/RNA recognition motif (RRM). Deletions which remove this region abolish the ability of eIF-4B to cooperate with eIF-4A in RNA binding and the ability to stimulate the helicase activity of eIF-4A. Results indicate that the carboxy-terminal RNA-binding region of eIF-4B is essential for eIF-4B function and is distinct from the RNP-CS/RRM (2). eIF4F binds to the mRNA cap structure and, in combination with eIF4B, is believed to unwind the secondary structure in the 5' untranslated region to facilitate ribosome binding. It has been suggested that eIF4B participates in mRNA-ribosome binding by acting as an intermediary between the mRNA and eIF3, via a direct interaction with the p170 subunit of eIF3 (3).


1. Milburn SC, et al. EMBO J, 9(9):2783-90, 1990
2. Methot N, et al. Mol Cell Biol 14(4):2307-16, 1994
3. Methot N, et al. Mol Cell Biol 16(10):5328-34, 1996

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Cat# AJ1254b
(40 western blots)
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