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eIF-4E Antibody (C-term)Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1255b | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
eIF-4E Antibody (C-term) - Product info | |
| Application | WB, IHC
|
| Primary Accession | P06730 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | Y449 |
| Calculated MW | 25097 Da |
| Gene ID 1977 | |
| Other Names EIF4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, mRNA cap-binding protein;eIF-4F 25 kDa subunit | |
| Target/Specificity A synthetic peptide corresponding to residues in the C-term of human eIF-4E, was used as immunogen. The antibody detects a band on western blot of approximately 28 kDa. | |
| Dilution WB~~1:1000~10000 IHC~~1:250~500 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions eIF-4E Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
eIF-4E Antibody (C-term) - Protein Information | |
| Name EIF4E | |
| Synonyms EIF4EL1, EIF4F | |
| Function Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap | |
| Cellular Location Cytoplasm, P-body. | |
eIF-4E Antibody (C-term) - Related products
AM1852a: EIF4E Antibody (Ascites)
AP1954a: EIF4E Antibody (N-term)
AP3474a: Phospho-EIF4E-S209 Antibody
RI11731: EIF4E predesign siRNA
BP3474a: Phospho-EIF4E-S209 Antibody Blocking Peptide
AJ1255b: eIF-4E Antibody (C-term)
AJ1255c: eIF-4E Antibody Phospho (pS209)
eIF-4E Antibody (C-term) - Application data
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A. Western blot analysis on 293 cell lysate using anti-eIF-4E RabMAb (C-term) (Cat. #AJ1255b).
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B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-eIF-4E (C-term) RabMAb (Cat. #AJ1255b).
eIF-4E Antibody (C-term) - Research Areas
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BACKGROUND
eIF-4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It exists in two forms: as a free form (25 kDa) and as part of a multiprotein complex eIF-4F (1). eIF-4E appears to be the least abundant of the initiation factors and acts as a rate-limiting step of initiation (2). Since translation is regulated by phosphorylation, eIF-4E phosphorylation at Ser 209 by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2 (Mnk2) may directly regulate the rate of protein synthesis initiation (3). There is also evidence that eIF-4E can function as an oncogene (4-5).
REFERENCES
1. Rychlik, et al Proc. Nat. Acad. Sci. 84: 945-949, 1987.
2. Rhoads, R. E., Joshi-Barve, S., and Rinker-Schaeffer, C.(1993) Prog. Nucleic Acids Res. Mol. Biol.46, 183-219
3. Gert C. Scheper, Nick A. Morrice, Miranda Kleijn and Christopher G. Proud Molec. Cell. Biol.; February 2001, p. 743-754, Vol. 21, No. 3
4. Jone et al. Molec. Cell. Biol. 16: 4754-4764, 1996.
5. Wendel et al. Nature 428: 332-337, 2004.
