Register or Login
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   eIF-4E Antibody Phospho (pS209)   

eIF-4E Antibody Phospho (pS209)

Rabbit Monoclonal Antibody

  • WB - eIF-4E Antibody Phospho (pS209) AJ1255c
    A. Western blot analysis on 293 cell lysates using anti-Phospho-eIF4E (pS209) RabMAb (Cat. #AJ1255c), 1:10,000 dilution. Cells were either (A) untreated (B) treated with AP
  • IHC - eIF-4E Antibody Phospho (pS209) AJ1255c
    B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Phospho-eIF4E (pS209) RabMAb (Cat. #AJ1255c).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P06730
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP2151Y
Calculated MW 25097 Da
Gene ID 1977
Other Names Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F
Target/Specificity A synthetic peptide corresponding to residues surrounding Serine 209 of human eIF4E was used as an immunogen.
Dilution WB~~1:1000~2000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionseIF-4E Antibody Phospho (pS209) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF4E
Synonyms EIF4EL1, EIF4F
Function Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Cellular Location Cytoplasm, P-body. Cytoplasm
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to, and receive a free "I Love Antibodies" mug.


eIF-4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It exists in two forms: as a free form (25 kDa) and as part of a multi-protein complex eIF-4F (1). eIF-4E appears to be the least abundant of the initiation factors and acts as a rate-limiting step of initiation (2). Since translation is regulated by phosphorylation, eIF-4E phosphorylation at Ser 209 by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2 (Mnk2) may directly regulate the rate of protein synthesis initiation (3). There is also evidence that eIF-4E can function as an oncogene (4-5)


1. Rychlik, et al Proc. Nat. Acad. Sci. 84: 945-949, 1987.
2. Rhoads, R. E., Joshi-Barve, S., and Rinker-Schaeffer, C.(1993) Prog. Nucleic Acids Res. Mol. Biol.46, 183-219
3. Gert C. Scheper, Nick A. Morrice, Miranda Kleijn and Christopher G. Proud Molec. Cell. Biol.; February 2001, p. 743-754, Vol. 21, No. 3
4. Jone et al. Molec. Cell. Biol. 16: 4754-4764, 1996.
5. Wendel et al. Nature 428: 332-337, 2004.

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at

Cat# AJ1255c
(40 western blots)
Alternative Products:

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Santa Cruz Alternative
Terms & Conditions