|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||16832 Da|
|Other Names||Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D, EIF5A|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-term of human eIF-5A was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||eIF-5A Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF- alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.|
|Cellular Location||Cytoplasm. Nucleus. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus, nuclear pore complex. Note=Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions|
|Tissue Location||Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
The human eukaryotic translation initiation factor 5A (eIF-5A) is an 18 kDa protein (1). eIF-5A precursor [(ec-eIF-5A(lys)] is the only cellular protein known to contain a specific lysine residue which is transformed into the unique amino acid hypusine [N-(4-amino-2-hydroxybutyl)-lysine] by a series of post-translational reactions (2). eIF-5A promotes the formation of the first peptide bond during the initial stage of protein synthesis (3).
1. Park,M.H., Chung,S.I., Cooper,H.L. and Folk,J.E. (1984) J. Biol. Chem., 259, 4563
2. Park,M.H., Cooper,H.L. and Folk,J.E. (1982) J. Biol. Chem., 257, 7219
3. Hershey,J.W.B. (1991) Annu. Rev. Biochem., 60, 717
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