- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
Elk-1 Antibody (C-term)Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AJ1257a | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Elk-1 Antibody (C-term) - Product info | |
| Application | WB
|
| Primary Accession | P19419 |
| Reactivity | Human |
| Clone Names | E277 |
| Calculated MW | 44888 Da |
| Gene ID 2002 | |
| Other Names ELK1, ETS domain-containing protein Elk-1 | |
| Target/Specificity A synthetic peptide corresponding to residues in C-terminus of human ELK-1 was used as immunogen. The antibody does not cross-react with other ETS family members. | |
| Dilution WB~~1:1000 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Elk-1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
Elk-1 Antibody (C-term) - Protein Information | |
| Name ELK1 | |
| Function Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element | |
| Cellular Location Nucleus. | |
| Tissue Location Lung and testis. | |
Elk-1 Antibody (C-term) - Related products
AP3309a: Phospho-ELK1-S383 Antibody
AP3377a: Phospho-ELK1-S389 Antibody
AP3424a: Phospho-ELK1-T417 Antibody
LY11846a: ELK1 Over-expression Lysate
BP3309a: Phospho-ELK1-S383 Antibody Blocking Peptide
BP3377a: Phospho-ELK1-S389 Antibody Blocking Peptide
BP3424a: Phospho-ELK1-T417 Antibody Blocking Peptide
BP6282a: ELK1 Antibody (S383) Blocking Peptide
Elk-1 Antibody (C-term) - Application data
-
A. Western blot analysis on A431 cell lysate using anti-Elk-1 (C-term) RabMAb (Cat. #AJ1257a), dilution 1:500.
Elk-1 Antibody (C-term) - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Elk-1 is a transcription factor that is part of the ets oncogene superfamily (1). Elk-1 forms a ternary complex with the serum response factors, SRE and SRF, mediating gene activity in response to serum and growth factors (2). Activation by several mitogen-activated protein kinases (Erk1 and Erk2) mediates the immediate early responses of the c-fos promoter to growth factors and other stimuli (3,4). Serines 383 and 389 in the C-terminal region are the preferred sites for phosphorylation-induced activation.
REFERENCES
1. Rao, V.N., et al. elk, tissue-specific ets-related genes on chromosomes X and 14 near translocation breakpoints. Science 244: 66
2. Marais, R., et al. The SRF accessory protein Elk-1 contains a growth factor-regulated transcriptional activation domain. Cell 73: 381
3. Harindranath, N., et al. The human elk-1 gene family: the functional gene and two processed pseudogenes embedded in the IgH locus. Gene 221: 215
4. Gille, H., et al. ERK phosphorylation potentiates Elk-1-mediated ternary complex formation and transactivation. EMBO J. 14: 951
