|Calculated MW||44888 Da|
|Other Names||ETS domain-containing protein Elk-1, ELK1|
|Target/Specificity||A synthetic peptide corresponding to residues in C-terminus of human ELK-1 was used as immunogen. The antibody does not cross-react with other ETS family members.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Elk-1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation (By similarity).|
|Tissue Location||Lung and testis.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Elk-1 is a transcription factor that is part of the ets oncogene superfamily (1). Elk-1 forms a ternary complex with the serum response factors, SRE and SRF, mediating gene activity in response to serum and growth factors (2). Activation by several mitogen-activated protein kinases (Erk1 and Erk2) mediates the immediate early responses of the c-fos promoter to growth factors and other stimuli (3,4). Serines 383 and 389 in the C-terminal region are the preferred sites for phosphorylation-induced activation.
1. Rao, V.N., et al. elk, tissue-specific ets-related genes on chromosomes X and 14 near translocation breakpoints. Science 244: 66
2. Marais, R., et al. The SRF accessory protein Elk-1 contains a growth factor-regulated transcriptional activation domain. Cell 73: 381
3. Harindranath, N., et al. The human elk-1 gene family: the functional gene and two processed pseudogenes embedded in the IgH locus. Gene 221: 215
4. Gille, H., et al. ERK phosphorylation potentiates Elk-1-mediated ternary complex formation and transactivation. EMBO J. 14: 951
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.