|Application ||WB, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||141468 Da|
|Other Names||Protein EMSY, EMSY, C11orf30|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-term of human EMSY isoform 1 was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EMSY Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulator which is able to repress transcription, possibly via its interaction with a multiprotein chromatin remodeling complex that modifies the chromatin. Its interaction with BRCA2 suggests that it may play a central role in the DNA repair function of BRCA2. As part of a histone H3-specific methyltransferase complex may mediate ligand-dependent transcriptional activation by nuclear hormone receptors.|
|Cellular Location||Nucleus. Note=Localizes to DNA damage markers in irradiated cells, suggesting that it participates in DNA repair process|
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Provided below are standard protocols that you may find useful for product applications.
EMSY is a novel protein whose sequence is found in a chromosomal region known to be involved in breast and ovarian cancer (1). The N-terminus of EMSY binds BRCA2 (exon 3). EMSY is capable of silencing the exon 3 encoded transcrip-tional activation domain of BRCA2. EMSY, just like BRCA2, is located in the nucleus and functions with DNA repair and transcriptional regulation factors (2). EMSY was found to be overactive in 13% of sporadic cases of breast cancer and 17% of ovarian cancers and it was also linked with poor survival, specifically in node-negative breast cancer, suggesting that it may be of prognostic value (1).
1. Hughes-Davies et al. Cell 115: 523-535, 2003
2. Pilch et al, Biochem. Cell Biol. 81, pp. 123
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