|Reactivity||Human, Mouse, Rat|
|Calculated MW||32620 Da|
|Other Names||Endonuclease G, mitochondrial, Endo G, 3130-, ENDOG|
|Target/Specificity||A synthetic peptide corresponding to residues on the C-terminus of human Endo G was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Endo G Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, also has ribonuclease (RNase) and RNase H activities. Capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
Endonuclease G (endoG) is a mitochondrion-specific nuclease that translocates to the nucleus during apoptosis. Once released from mitochondria, endoG cleaves chromatin DNA into nucleosomal fragments independently of caspases. Therefore, endoG represents a caspase-independent apoptotic pathway initiated from the mitochondria (1). Endonuclease G (EndoG) is a nuclear-encoded mitochondrial protein reported to be important for both nuclear DNA fragmentation during apoptosis and mitochondrial DNA replication. Findings indicate that EndoG is essential during early embryogenesis and plays a critical role in normal apoptosis and nuclear DNA fragmentation (2).
1. Li LY, et al. Nature 412(6842):95-9, 2001
2. Zhang J, et al. Proc Natl Acad Sci, 100(26):15782-7, 2003.
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