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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Enolase-2 Antibody gamma (NSE)   

Enolase-2 Antibody gamma (NSE)

Rabbit Monoclonal Antibody

  • WB - Enolase-2 Antibody gamma (NSE) AJ1261a
    A. Western blot analysis on (A) fetal brain, (B) SH-SY-5Y, (C) HeLa and (D) Y79 cell lysates using anti-Enolase 2 gamma (NSE) RabMAb (Cat. #AJ1261a), dilution: 1:20,000.
  • IHC - Enolase-2 Antibody gamma (NSE) AJ1261a
    B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Enolase 2 gamme (NSE) RabMAb (Cat. #AJ1261a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09104
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR3377
Calculated MW 47269 Da
Gene ID 2026
Other Names Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2
Target/Specificity A synthetic peptide corresponding to residues in human Enolase 2 gamma (NSE) was used as an immunogen.
Dilution WB~~1:500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEnolase-2 Antibody gamma (NSE) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ENO2
Function Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
Cellular Location Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
Tissue Location The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons
Research Areas
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Enolase is a glycolytic enzyme that catalyzes the interconversion of 2-phosphoenolpyruvate to phosphoenolpyruvate in the glycolytic pathway (1, 2). The functional enzyme is a dimer made up of subunits referred to as alpha, beta, and gamma. The alpha-, or nonneuronal, enolase (ENO1) is a nearly ubiquitous form, found in almost all tissues, and its expression precedes that of the other isoforms in the early stage of embryonic development. The beta-, or muscle-specific, enolase (ENO3) is present in adult skeletal muscle, and the gamma-, or neuron-specific, enolase (ENO2) is the major form found in mature neurons and in cells of neuronal origin (2).


1. H. Yamamoto, et al. Acta Cryst. 64(11), 1087-1090, 2008
2. Grzeschik KH., et al. Cytogenet Cell Genet. 16(1-5):142-8, 1976

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Cat# AJ1261a
(40 western blots)
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